LOCUS       sequence-10424-e        9106 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9106)
  AUTHORS   Jackson EL, Willis N, Mercer K, Bronson RT, Crowley D, Montoya R, 
            Jacks T, Tuveson DA
  TITLE     Analysis of lung tumor initiation and progression using conditional 
            expression of oncogenic K-ras.
  JOURNAL   Genes Dev. 2001 Dec 15. 15(24):3243-8.
  PUBMED    11751630
REFERENCE   2  (bases 1 to 9106)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Genes Dev. 
            2001 Dec 15. 15(24):3243-8."
COMMENT     Sequence Label: Fret-Stop-Fret TOPO
FEATURES             Location/Qualifiers
     source          1..9106
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    107..128
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(143..148,149..166,167..173)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    181..197
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     186..208
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     205..221
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     205..221
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     promoter        239..257
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     239..256
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     protein_bind    complement(310..343)
                     /label=FRT (minimal)
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="supports FLP-mediated excision but not integration 
                     (Turan and Bode, 2011)"
     regulatory      371..380
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     polyA_signal    405..526
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     CDS             complement(951..971)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     intron          1101..1166
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     polyA_signal    1259..1380
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     CDS             complement(1805..1825)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     intron          1955..2020
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     polyA_signal    2113..2234
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     CDS             complement(2659..2679)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     intron          2809..2874
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     polyA_signal    2967..3088
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     CDS             complement(3513..3533)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     intron          3663..3728
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     promoter        4338..4837
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(4373..4396)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     4770..4789
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     CDS             4881..5480
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(4881..4900)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     5377..5397
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     protein_bind    complement(5913..5946)
                     /label=FRT (minimal)
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="supports FLP-mediated excision but not integration 
                     (Turan and Bode, 2011)"
     primer_bind     complement(5993..6012)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(5994..6012)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(6019..6036)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(6019..6035)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6028..6050)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     CDS             6173..6475
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="QFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDKV
                     SRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     primer_bind     6354..6373
                     /label=ccdB-fwd
                     /note="ccdB gene, forward primer"
     CDS             6824..7618
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             6824..7618
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(6878..6897)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     7488..7507
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     regulatory      7819..7828
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             7825..8199
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     rep_origin      8337..8925
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8826..8845
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     9079..9096
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 agcgcccaat acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcagctggc
       61 acgacaggtt tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat gtgagttagc
      121 tcactcatta ggcaccccag gctttacact ttatgcttcc ggctcgtatg ttgtgtggaa
      181 ttgtgagcgg ataacaattt cacacaggaa acagctatga ccatgattac gccaagctat
      241 ttaggtgaca ctatagaata ctcaagctat gcatcaagct tggtaccgag ctcggatccg
      301 tcgacgtttg aagttcctat actttctaga gaataggaac ttcgtttctc gagctgcaga
      361 cttactcaag ccatggtggc tagcttgcgg aattccagac atgataagat acattgatga
      421 gtttggacaa accacaacta gaatgcagtg aaaaaaatgc tttatttgtg aaatttgtga
      481 tgctattgct ttatttgtaa ccattataag ctgcaataaa caagttaaca acaacaattg
      541 cattcatttt atgtttcagg ttcaggggga ggtgtgggag gttttttaaa gcaagtaaaa
      601 cctctacaaa tgtggtatgg ctgattatga tctctagtca aggcactata catcaaatat
      661 tccttattaa cccctttaca aattaaaaag ctaaaggtac acaatttttg agcatagtta
      721 ttaatagcag acactctatg cctgtgtgga gtaagaaaaa acagtatgtt atgattataa
      781 ctgttatgcc tacttataaa ggttacagaa tatttttcca taattttctt gtatagcagt
      841 gcagcttttt cctttgtggt gtaaatagca aagcaagcaa gagttctatt actaaacaca
      901 gcatgactca aaaaacttag caattctgaa ggaaagtcct tggggtcttc tacctttctc
      961 ttcttttttg gaggagtaga atgttgagag tcagcagtag cctcatcatc actagatggc
     1021 atttcttctg agcaaaacag gttttcctca ttaaaggcat tccaccactg ctcccattca
     1081 tcagttccat aggttggaat ctaaaataca caaacaatta gaatcagtag tttaacacat
     1141 tatacactta aaaattttat atttacctta gagctttaaa tctctgtagg tagtttgtcc
     1201 aattatgtca caccacagaa gtaaggttcc ttcacaaaga tccctcgacc agacatgata
     1261 agatacattg atgagtttgg acaaaccaca actagaatgc agtgaaaaaa atgctttatt
     1321 tgtgaaattt gtgatgctat tgctttattt gtaaccatta taagctgcaa taaacaagtt
     1381 aacaacaaca attgcattca ttttatgttt caggttcagg gggaggtgtg ggaggttttt
     1441 taaagcaagt aaaacctcta caaatgtggt atggctgatt atgatctcta gtcaaggcac
     1501 tatacatcaa atattcctta ttaacccctt tacaaattaa aaagctaaag gtacacaatt
     1561 tttgagcata gttattaata gcagacactc tatgcctgtg tggagtaaga aaaaacagta
     1621 tgttatgatt ataactgtta tgcctactta taaaggttac agaatatttt tccataattt
     1681 tcttgtatag cagtgcagct ttttcctttg tggtgtaaat agcaaagcaa gcaagagttc
     1741 tattactaaa cacagcatga ctcaaaaaac ttagcaattc tgaaggaaag tccttggggt
     1801 cttctacctt tctcttcttt tttggaggag tagaatgttg agagtcagca gtagcctcat
     1861 catcactaga tggcatttct tctgagcaaa acaggttttc ctcattaaag gcattccacc
     1921 actgctccca ttcatcagtt ccataggttg gaatctaaaa tacacaaaca attagaatca
     1981 gtagtttaac acattataca cttaaaaatt ttatatttac cttagagctt taaatctctg
     2041 taggtagttt gtccaattat gtcacaccac agaagtaagg ttccttcaca aagatccctc
     2101 gaccagacat gataagatac attgatgagt ttggacaaac cacaactaga atgcagtgaa
     2161 aaaaatgctt tatttgtgaa atttgtgatg ctattgcttt atttgtaacc attataagct
     2221 gcaataaaca agttaacaac aacaattgca ttcattttat gtttcaggtt cagggggagg
     2281 tgtgggaggt tttttaaagc aagtaaaacc tctacaaatg tggtatggct gattatgatc
     2341 tctagtcaag gcactataca tcaaatattc cttattaacc cctttacaaa ttaaaaagct
     2401 aaaggtacac aatttttgag catagttatt aatagcagac actctatgcc tgtgtggagt
     2461 aagaaaaaac agtatgttat gattataact gttatgccta cttataaagg ttacagaata
     2521 tttttccata attttcttgt atagcagtgc agctttttcc tttgtggtgt aaatagcaaa
     2581 gcaagcaaga gttctattac taaacacagc atgactcaaa aaacttagca attctgaagg
     2641 aaagtccttg gggtcttcta cctttctctt cttttttgga ggagtagaat gttgagagtc
     2701 agcagtagcc tcatcatcac tagatggcat ttcttctgag caaaacaggt tttcctcatt
     2761 aaaggcattc caccactgct cccattcatc agttccatag gttggaatct aaaatacaca
     2821 aacaattaga atcagtagtt taacacatta tacacttaaa aattttatat ttaccttaga
     2881 gctttaaatc tctgtaggta gtttgtccaa ttatgtcaca ccacagaagt aaggttcctt
     2941 cacaaagatc cctcgaccag acatgataag atacattgat gagtttggac aaaccacaac
     3001 tagaatgcag tgaaaaaaat gctttatttg tgaaatttgt gatgctattg ctttatttgt
     3061 aaccattata agctgcaata aacaagttaa caacaacaat tgcattcatt ttatgtttca
     3121 ggttcagggg gaggtgtggg aggtttttta aagcaagtaa aacctctaca aatgtggtat
     3181 ggctgattat gatctctagt caaggcacta tacatcaaat attccttatt aaccccttta
     3241 caaattaaaa agctaaaggt acacaatttt tgagcatagt tattaatagc agacactcta
     3301 tgcctgtgtg gagtaagaaa aaacagtatg ttatgattat aactgttatg cctacttata
     3361 aaggttacag aatatttttc cataattttc ttgtatagca gtgcagcttt ttcctttgtg
     3421 gtgtaaatag caaagcaagc aagagttcta ttactaaaca cagcatgact caaaaaactt
     3481 agcaattctg aaggaaagtc cttggggtct tctacctttc tcttcttttt tggaggagta
     3541 gaatgttgag agtcagcagt agcctcatca tcactagatg gcatttcttc tgagcaaaac
     3601 aggttttcct cattaaaggc attccaccac tgctcccatt catcagttcc ataggttgga
     3661 atctaaaata cacaaacaat tagaatcagt agtttaacac attatacact taaaaatttt
     3721 atatttacct tagagcttta aatctctgta ggtagtttgt ccaattatgt cacaccacag
     3781 aagtaaggtt ccttcacaaa gatccctcga tcgagaaaaa aaatataaaa gagatggagg
     3841 aacgggaaaa agttagttgt ggtgataggt ggcaagtggt attccgtaag aacaacaaga
     3901 aaagcatttc atattatggc tgaactgagc gaacaagtgc aaaatttaag catcaacgac
     3961 aacaacgaga atggttatgt tcctcctcac ttaagaggaa aaccaagaag tgccagaaat
     4021 aacatgagca actacaataa caacaacggc ggctacaacg gtggccgtgg cggtggcagc
     4081 ttctttagca acaaccgtcg tggtggttac ggcaacggtg gtttcttcgg tggaaacaac
     4141 ggtggcagca gatctaacgg ccgttctggt ggtagatgga tcgatggcaa acatgtccca
     4201 gctccaagaa acgaaaaggc cgagatcgcc atatttggtg tccccgagga tccctgtgga
     4261 aaaaaaaggg acaggataag tatgacatca tcaaggaaac cctggactac tgcgcccttc
     4321 gacctcgaaa ttctaccggg taggggaggc gcttttccca aggcagtctg gagcatgcgc
     4381 tttagcagcc ccgctgggca cttggcgcta cacaagtggc ctctggcctc gcacacattc
     4441 cacatccacc ggtaggcgcc aaccggctcc gttctttggt ggccccttcg cgccaccttc
     4501 tactcctccc ctagtcagga agttcccccc cgccccgcag ctcgcgtcgt gcaggacgtg
     4561 acaaatggaa gtagcacgtc tcactagtct cgtgcagatg gacagcaccg ctgagcaatg
     4621 gaagcgggta ggcctttggg gcagcggcca atagcagctt tgctccttcg ctttctgggc
     4681 tcagaggctg ggaaggggtg ggtccggggg cgggctcagg ggcgggctca ggggcggggc
     4741 gggcgcccga aggtcctccg gaggcccggc attctgcacg cttcaaaagc gcacgtctgc
     4801 cgcgctgttc tcctcttcct catctccggg cctttcgacc tgcatccatc tagatctcga
     4861 tcgagcagct gaagcttacc atgaccgagt acaagcccac ggtgcgcctc gccacccgcg
     4921 acgacgtccc cagggccgta cgcaccctcg ccgccgcgtt cgccgactac cccgccacgc
     4981 gccacaccgt cgatccggac cgccacatcg agcgggtcac cgagctgcaa gaactcttcc
     5041 tcacgcgcgt cgggctcgac atcggcaagg tgtgggtcgc ggacgacggc gccgcggtgg
     5101 cggtctggac cacgccggag agcgtcgaag cgggggcggt gttcgccgag atcggcccgc
     5161 gcatggccga gttgagcggt tcccggctgg ccgcgcagca acagatggaa ggcctcctgg
     5221 cgccgcaccg gcccaaggag cccgcgtggt tcctggccac cgtcggcgtc tcgcccgacc
     5281 accagggcaa gggtctgggc agcgccgtcg tgctccccgg agtggaggcg gccgagcgcg
     5341 ccggggtgcc cgccttcctg gagacctccg cgccccgcaa cctccccttc tacgagcggc
     5401 tcggcttcac cgtcaccgcc gacgtcgagg tgcccgaagg accgcgcacc tggtgcatga
     5461 cccgcaagcc cggtgcctga cgcccgcccc acgacccgca gcgcccgacc gaaaggagcg
     5521 cacgacccca tgcatcgatg atatcagatc cccgggatgc agaaattgat gatctattaa
     5581 acaataaaga tgtccactaa aatggaagtt tttcctgtca tactttgtta agaagggtga
     5641 gaacagagta cctacatttt gaatggaagg attggagcta cgggggtggg ggtggggtgg
     5701 gattagataa atgcctgctc tttactgaag gctctttact attgctttat gataatgttt
     5761 catagttgga tatcataatt taaacaagca aaaccaaatt aagggccagc tcattcctcc
     5821 cactcatgat ctatagatct atagatctct cgtgggatca ttgtttttct cttgattccc
     5881 actttgtggt tctaagtaac ggctcgaggt ttgaagttcc tatactttct agagaatagg
     5941 aacttcgttg tcgacgcggc cgctcgagca tgcatctaga gggcccaatt cgccctatag
     6001 tgagtcgtat tacaattcac tggccgtcgt tttacaacgt cgtgactggg aaaaccctgg
     6061 cgttacccaa cttaatcgcc ttgcagcaca tccccctttc gccagctggc gtaatagcga
     6121 agaggcccgc accgatcgcc cttcccaaca gttgcgcagc ctatacgtac ggcagtttaa
     6181 ggtttacacc tataaaagag agagccgtta tcgtctgttt gtggatgtac agagtgatat
     6241 tattgacacg ccggggcgac ggatggtgat ccccctggcc agtgcacgtc tgctgtcaga
     6301 taaagtctcc cgtgaacttt acccggtggt gcatatcggg gatgaaagct ggcgcatgat
     6361 gaccaccgat atggccagtg tgccggtctc cgttatcggg gaagaagtgg ctgatctcag
     6421 ccaccgcgaa aatgacatca aaaacgccat taacctgatg ttctggggaa tataaatgtc
     6481 aggcatgaga ttatcaaaaa ggatcttcac ctagatcctt ttcacgtaga aagccagtcc
     6541 gcagaaacgg tgctgacccc ggatgaatgt cagctactgg gctatctgga caagggaaaa
     6601 cgcaagcgca aagagaaagc aggtagcttg cagtgggctt acatggcgat agctagactg
     6661 ggcggtttta tggacagcaa gcgaaccgga attgccagct ggggcgccct ctggtaaggt
     6721 tgggaagccc tgcaaagtaa actggatggc tttctcgccg ccaaggatct gatggcgcag
     6781 gggatcaagc tctgatcaag agacaggatg aggatcgttt cgcatgattg aacaagatgg
     6841 attgcacgca ggttctccgg ccgcttgggt ggagaggcta ttcggctatg actgggcaca
     6901 acagacaatc ggctgctctg atgccgccgt gttccggctg tcagcgcagg ggcgcccggt
     6961 tctttttgtc aagaccgacc tgtccggtgc cctgaatgaa ctgcaagacg aggcagcgcg
     7021 gctatcgtgg ctggccacga cgggcgttcc ttgcgcagct gtgctcgacg ttgtcactga
     7081 agcgggaagg gactggctgc tattgggcga agtgccgggg caggatctcc tgtcatctca
     7141 ccttgctcct gccgagaaag tatccatcat ggctgatgca atgcggcggc tgcatacgct
     7201 tgatccggct acctgcccat tcgaccacca agcgaaacat cgcatcgagc gagcacgtac
     7261 tcggatggaa gccggtcttg tcgatcagga tgatctggac gaagagcatc aggggctcgc
     7321 gccagccgaa ctgttcgcca ggctcaaggc gagcatgccc gacggcgagg atctcgtcgt
     7381 gacccatggc gatgcctgct tgccgaatat catggtggaa aatggccgct tttctggatt
     7441 catcgactgt ggccggctgg gtgtggcgga ccgctatcag gacatagcgt tggctacccg
     7501 tgatattgct gaagagcttg gcggcgaatg ggctgaccgc ttcctcgtgc tttacggtat
     7561 cgccgctccc gattcgcagc gcatcgcctt ctatcgcctt cttgacgagt tcttctgaat
     7621 tattaacgct tacaatttcc tgatgcggta ttttctcctt acgcatctgt gcggtatttc
     7681 acaccgcata caggtggcac ttttcgggga aatgtgcgcg gaacccctat ttgtttattt
     7741 ttctaaatac attcaaatat gtatccgctc atgagacaat aaccctgata aatgcttcaa
     7801 taatagcacg tgaggagggc caccatggcc aagttgacca gtgccgttcc ggtgctcacc
     7861 gcgcgcgacg tcgccggagc ggtcgagttc tggaccgacc ggctcgggtt ctcccgggac
     7921 ttcgtggagg acgacttcgc cggtgtggtc cgggacgacg tgaccctgtt catcagcgcg
     7981 gtccaggacc aggtggtgcc ggacaacacc ctggcctggg tgtgggtgcg cggcctggac
     8041 gagctgtacg ccgagtggtc ggaggtcgtg tccacgaact tccgggacgc ctccgggccg
     8101 gccatgaccg agatcggcga gcagccgtgg gggcgggagt tcgccctgcg cgacccggcc
     8161 ggcaactgcg tgcacttcgt ggccgaggag caggactgac acgtgctaaa acttcatttt
     8221 taatttaaaa ggatctaggt gaagatcctt tttgataatc tcatgaccaa aatcccttaa
     8281 cgtgagtttt cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga
     8341 gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg
     8401 gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc
     8461 agagcgcaga taccaaatac tgtccttcta gtgtagccgt agttaggcca ccacttcaag
     8521 aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc
     8581 agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg
     8641 cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac
     8701 accgaactga gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga
     8761 aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt
     8821 ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag
     8881 cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg
     8941 gcctttttac ggttcctggg cttttgctgg ccttttgctc acatgttctt tcctgcgtta
     9001 tcccctgatt ctgtggataa ccgtattacc gcctttgagt gagctgatac cgctcgccgc
     9061 agccgaacga ccgagcgcag cgagtcagtg agcgaggaag cggaag
//
