LOCUS       sequence-104247-         705 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 705)
  AUTHORS   Schatz PJ, Solomon F, Botstein D
  TITLE     Genetically essential and nonessential alpha-tubulin genes specify 
            functionally interchangeable proteins.
  JOURNAL   Mol Cell Biol 1986 Nov;6(11):3722-33.
  PUBMED    3540600
REFERENCE   2  (bases 1 to 705)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Cell 
            Biol"; date: "1986-11"; volume: "6(11)"; pages: "3722-33"
COMMENT     Sequence Label: pRB306
FEATURES             Location/Qualifiers
     source          1..705
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        complement(191..295)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     363..381
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        join(408..413,414..430,431..436)
                     /label=tet promoter
                     /note="E. coli promoter for tetracycline efflux protein 
                     gene"
ORIGIN
        1 aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc agttcgatgt
       61 aacccactcg tgcacccaac tgatcttcag catcttttac tttcaccagc gtttctgggt
      121 gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt
      181 gaatactcat actcttcctt tttcaatatt attgaagcat ttatcagggt tattgtctca
      241 tgagcggata catatttgaa tgtatttaga aaaataaaca aataggggtt ccgcgcacat
      301 ttccccgaaa agtgccacct gacgtctaag aaaccattat tatcatgaca ttaacctata
      361 aaaataggcg tatcacgagg ccctttcgtc ttcaagaatt ctcatgtttg acagcttatc
      421 atcgataagc tttaatgcgg tagtttatca cagttaaatt gctaacgcag tcaggcaccg
      481 tgtatgaaat ctaacaatgc gctcatcgtc atcctcggca ccgtcaccct ggatgctgta
      541 ggcataggct tggttatgcc ggtactgccg ggcctcttgc gggatatcgt ccattccgac
      601 agcatcgcca gtcactatgg cgtgctgcta gcgctatatg cgttgatgca atttctatgc
      661 gcacccgttc tcggagcact gtccgaccgc tttggccgcc gccca
//
