LOCUS       sequence-104249-         697 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses human OTULIN (full-length) in E. coli..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 697)
  AUTHORS   Keusekotten K, Elliott PR, Glockner L, Fiil BK, Damgaard RB, Kulathu
            Y, Wauer T, Hospenthal MK, Gyrd-Hansen M, Krappmann D, Hofmann K, 
            Komander D
  TITLE     OTULIN antagonizes LUBAC signaling by specifically hydrolyzing 
            Met1-linked polyubiquitin.
  JOURNAL   Cell. 2013 Jun 6;153(6):1312-26. doi: 10.1016/j.cell.2013.05.014.
  PUBMED    23746843
REFERENCE   2  (bases 1 to 697)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2013.05"; journalName: "Cell"; date: "2013-06-6- 6";
            volume: "153"; issue: "6"; pages: "1312-26"
COMMENT     Sequence Label: pOPINB-OTULIN (full-length, aa 1-352)
FEATURES             Location/Qualifiers
     source          1..697
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     44..63
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        44..62
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    63..87
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             102..124
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             143..160
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             170..193
                     /codon_start=1
                     /product="recognition and cleavage site for human 
                     rhinovirus 3C and PreScission proteases"
                     /label=HRV 3C site
                     /translation="LEVLFQGP"
ORIGIN
        1 gatgcgtccg gcgtagagga tcgagatctc gatcccgcga aattaatacg actcactata
       61 ggggaattgt gagcggataa caattcccct ctagaaataa ttttgtttaa ctttaagaag
      121 gagatatacc atgggcagca gccatcatca tcatcatcac agcagcggtc tggaagttct
      181 gtttcagggc ccgatgagtc gggggactat gccccagccc gaagcgtggc caggcgcgag
      241 ctgcgccgag acgccggcgc gggaggcggc ggccacggcg cgggacggcg ggaaggcggc
      301 ggccagcggg cagccgcggc ccgagatgca gtgcccggcc gagcatgagg aggacatgta
      361 ccgtgctgca gatgaaatag aaaaggagaa agaattgctt atacatgaaa gaggggcatc
      421 agaaccgaga ttaagcgtag ctcctgaaat ggatatcatg gactactgca aaaaagaatg
      481 gagaggaaat acacagaaag caacgtgtat gaaaatgggc tatgaagagg tttctcagaa
      541 gttcacctcc atacggcgag tccgtggtga taattactgt gcactgaggg ccacgctgtt
      601 ccaggccatg agccaggctg tggggctgcc gccctggctg caggacccgg agctcatgct
      661 gttaccagaa aaactcataa gcaaatacaa ctggatc
//
