LOCUS       sequence-104268-         758 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  AAV vector with CaMKIIa promoter, EGFP, WPRE, and bGHpA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 758)
  AUTHORS   Choi JH, Yu NK, Baek GC, Bakes J, Seo D, Nam HJ, Baek SH, Lim CS, 
            Lee YS, Kaang BK
  TITLE     Optimization of AAV expression cassettes to improve packaging 
            capacity and transgene expression in neurons.
  JOURNAL   Mol Brain. 2014 Mar 11;7:17. doi: 10.1186/1756-6606-7-17.
  PUBMED    24618276
REFERENCE   2  (bases 1 to 758)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol 
            Brain."; date: "2014-03-11"; volume: "7:17. doi"; pages: " 
            10.1186/1756-6606-7-17"
COMMENT     Sequence Label: pAAV-CWB-EGFP
FEATURES             Location/Qualifiers
     source          1..758
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     misc_feature    66..654
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     CDS             172..183
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     581..601
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     primer_bind     complement(722..743)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 ggaaggcacg ggggaggggc aaacaacaga tggctggcaa ctagaaggca caggatcccc
       61 ccgatgcggg gaggcggccc aaagggagat ccgactcgtc tgagggcgaa ggcgaagacg
      121 cggaagaggc cgcagagccg gcagcaggcc gcgggaagga aggtccgctg gattgagggc
      181 cgaagggacg tagcagaagg acgtcccgcg cagaatccag gtggcaacac aggcgagcag
      241 ccaaggaaag gacgatgatt tccccgacaa caccacggaa ttgtcagtgc ccaacagccg
      301 agcccctgtc cagcagcggg caaggcaggc ggcgatgagt tccgccgtgg caatagggag
      361 ggggaaagcg aaagtcccgg aaaggagctg acaggtggtg gcaatgcccc aaccagtggg
      421 ggttgcgtca gcaaacacag tgcacaccac gccacgttgc ctgacaacgg gccacaactc
      481 ctcataaaga gacagcaacc aggatttata caaggaggag aaaatgaaag ccatacggga
      541 agcaatagca tgatacaaag gcattaaagc agcgtatcca catagcgtaa aaggagcaac
      601 atagttaaga ataccagtca atctttcaca aattttgtaa tccagaggtt gattatcggg
      661 gctgcaggaa ttcgatttac ttgtacagct cgtccatgcc gagagtgatc ccggcggcgg
      721 tcacgaactc cagcaggacc atgtgatcgc gcttctcg
//
