LOCUS       sequence-104269-         659 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Inserts a puromycin resistance gene and a VSG117 gene into the 
            VSG221 expression site of T.brucei downstream of the expression site
            promoter..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 659)
  AUTHORS   Smith TK, Vasileva N, Gluenz E, Terry S, Portman N, Kramer S, 
            Carrington M, Michaeli S, Gull K, Rudenko G
  TITLE     Blocking variant surface glycoprotein synthesis in Trypanosoma 
            brucei triggers a general arrest in translation initiation.
  JOURNAL   PLoS One. 2009 Oct 26;4(10):e7532. doi: 
            10.1371/journal.pone.0007532.
  PUBMED    19855834
REFERENCE   2  (bases 1 to 659)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2009-10-26"; volume: "4(10):e7532. doi"; pages: " 
            10.1371/journal.pone.0007532"
COMMENT     Sequence Label: p221- purVSG117UTR
FEATURES             Location/Qualifiers
     source          1..659
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 tcttgttttc tcgaccagcc ctactagagg agaaagaata gtaacccttt catcaaagaa
       61 aatagtcgaa gcttatggac tgccatacaa aggagacact aggggtcaca caatggaggc
      121 gatcaacgat gctaacacta tcactgcttt acgccatcac tccagcggac ggcgccaaag
      181 aagcccttga atacaaaact tggacaaacc actgcggact ggcggccaca ctgagaaagg
      241 ttgccggtgg agtattaacg aaactgaaaa gccacattag ctaccggaaa aaactggaag
      301 aaatggaaac gaagctacga atctacgcac taaaaggaga cggagtggga gagcaaaaat
      361 cagcggagat actagcaaca acggcagccc taatgcgaca aaaagcactc acaccagaag
      421 aagcaaattt gaaaacagcg ctgaaggcgg caggattcgc aggcgaagga gcggcagccg
      481 tcagcagcta cctgatgaca ctcgggacac tgacaacaag cggatctgcg cactgcctaa
      541 gcaacgaagg cggcgacggt gacggaaaag accaacttgc gccgaaaggc tgccggcacg
      601 gcacagaagc agacttcgac gcaggagccg gcccggcaga atctgaagta gccgacagc
//
