LOCUS       sequence-104270-         687 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Inserts a puromycin resistance gene and a VSG117 gene into the 
            VSG221 expression site of T.brucei downstream of the expression site
            promoter..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 687)
  AUTHORS   Smith TK, Vasileva N, Gluenz E, Terry S, Portman N, Kramer S, 
            Carrington M, Michaeli S, Gull K, Rudenko G
  TITLE     Blocking variant surface glycoprotein synthesis in Trypanosoma 
            brucei triggers a general arrest in translation initiation.
  JOURNAL   PLoS One. 2009 Oct 26;4(10):e7532. doi: 
            10.1371/journal.pone.0007532.
  PUBMED    19855834
REFERENCE   2  (bases 1 to 687)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2009-10-26"; volume: "4(10):e7532. doi"; pages: " 
            10.1371/journal.pone.0007532"
COMMENT     Sequence Label: p221- purVSG117UTR
FEATURES             Location/Qualifiers
     source          1..687
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             277..285
                     /label=Shine-Dalgarno sequence
                     /note="full consensus sequence for ribosome-binding sites 
                     upstream of start codons in E. coli; complementary to a 
                     region in the 3' end of the 16S rRNA (Chen et al., 1994)"
ORIGIN
        1 tttgaggggg gaaagggctg caggaattcc taaaaaagca aggccgcaaa cgcagcagca
       61 ctgagggcga attttttggt tactagaata gaggaatctt tgcaagcatt attttcccat
      121 ttgcagttgc tctccttctt gcaggtatct cccaatttcc ctttgcattt atctgttgtc
      181 gcttcggttc ctcctgcagt ttgagtttgt gttacagaga cccccttttc ttttgctttt
      241 gttgagttaa atttgcagtg cttttctccc gctttaacct ccttatgcca actgcatatc
      301 ttgtcctcgt tgcatttggg ttcctcagat attttattgc actcgctttc aggggatttg
      361 cctttttgat ctgctagttc ggtttcgagt ttagttattt tctccgctgt ttttttctgt
      421 tcttctttgt tgaccgtgta ataataactt aaaagttttt gtagctcggc cgtgtctgtg
      481 actgttccta actctttcgt gctgcctttg gtcgccgcca ggtcaactat attctgtcct
      541 ttgattttag tccagagcgg ctctagtttg ttgttgttgg ttccgaaata attgtttttc
      601 tcggtggcca ggtcgatcgt cacttcctct ggtccttttt gctgtgatgc tgccgcttct
      661 agtttcagta tttccgccat ctccgga
//
