LOCUS       sequence-104280-         680 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  a ligation-independent cloning (LIC) retroviral vector for stable 
            gene transduction in mammalian cells. Puro selection. .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 680)
  AUTHORS   Patel A, Munoz A, Halvorsen K, Rai P
  TITLE     Creation and validation of a ligation-independent cloning (LIC) 
            retroviral vector for stable gene transduction in mammalian cells.
  JOURNAL   BMC Biotechnol. 2012 Jan 16;12:3. doi: 10.1186/1472-6750-12-3.
  PUBMED    22248071
REFERENCE   2  (bases 1 to 680)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "BMC 
            Biotechnol."; date: "2012-01-16"; volume: "12:3. doi"; pages: " 
            10.1186/1472-6750-12-3"
COMMENT     Sequence Label: pBLIC-puro
FEATURES             Location/Qualifiers
     source          1..680
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(18..38)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        23..352
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      203..338
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(362..381)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
ORIGIN
        1 tgtgagctcg accctgtgga atgtgtgtca gttagggtgt ggaaagtccc caggctcccc
       61 agcaggcaga agtatgcaaa gcatgcatct caattagtca gcaaccaggt gtggaaagtc
      121 cccaggctcc ccagcaggca gaagtatgca aagcatgcat ctcaattagt cagcaaccat
      181 agtcccgccc ctaactccgc ccatcccgcc cctaactccg cccagttccg cccattctcc
      241 gccccatggc tgactaattt tttttattta tgcagaggcc gaggccgcct cggcctctga
      301 gctattccag aagtagtgag gaggcttttt tggaggccta ggcttttgca aaaagcttac
      361 catgaccgag tacaagccca cggtgcgcct cgccacccgc gacgacgtcc ccagggccgt
      421 acgcaccctc gccgccgcgt tcgccgacta ccccgccacg cgccacaccg tcgatccgga
      481 ccgccacatc gagcgggtca ccgagctgca agaactcttc ctcacgcgcg tcgggctcga
      541 catcggcaag gtgtgggtcg cggacgacgg cgccgcggtg gcggtctgga ccacgccgga
      601 gagcgtcgaa gcgggggcgg tgttcgccga gatcggcccg cgcatggccg agttgagcgg
      661 ttcccggctg gccgcgcagc
//
