LOCUS       sequence-104282-         678 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  a ligation-independent cloning (LIC) retroviral vector for stable 
            gene transduction in mammalian cells. Puro selection. .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 678)
  AUTHORS   Patel A, Munoz A, Halvorsen K, Rai P
  TITLE     Creation and validation of a ligation-independent cloning (LIC) 
            retroviral vector for stable gene transduction in mammalian cells.
  JOURNAL   BMC Biotechnol. 2012 Jan 16;12:3. doi: 10.1186/1472-6750-12-3.
  PUBMED    22248071
REFERENCE   2  (bases 1 to 678)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "BMC 
            Biotechnol."; date: "2012-01-16"; volume: "12:3. doi"; pages: " 
            10.1186/1472-6750-12-3"
COMMENT     Sequence Label: pBLIC-puro
FEATURES             Location/Qualifiers
     source          1..678
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(25..441)
                     /label=gag (truncated)
                     /note="truncated Moloney murine leukemia virus (MMLV) gag 
                     gene lacking the start codon"
     primer_bind     complement(41..57)
                     /label=pBABE 5'
                     /note="Psi packaging signal, forward primer for pBABE 
                     vectors"
     primer_bind     complement(73..95)
                     /label=pLXSN 5'
                     /note="Murine stem cell virus, forward primer. Also called 
                     MSCV"
ORIGIN
        1 gtgagatggt gtgcggatcc ggccggcgcc tagagaagga gtgagggctg gataaaggga
       61 ggatcgaggc ggggtcgaac gaggaggttc aagggggaga gacggggcgg atggaggaag
      121 aggaggcgga ggcttagggt gtacaaaggg cttgacccag ggaggggggt caaaagccaa
      181 ggcttcccag gtcacgatgt aggggacctg gtctgggtgt ccatgcgggc caggtgaaaa
      241 gaccttgatc ttaacctggg tgatgaggtc tcggttaaag gtgccgtctc gcggccatcc
      301 gacgttaaag gttggccatt ctgcagagca gaaggtaacc caacgtctct tcttgacatc
      361 taccgactgg ttgtgagcga tccgctcgac atctttccag tgacctaagg tcaaacttaa
      421 gggagtggta acagtctggc cctaattttc agacaaatac agaaacacag tcagacagag
      481 acaacacaga acgatgctgc agcgctgcag cagacaagac gcgcggcttc ggttccaaac
      541 cgaaagcaaa aattcagacg gaggcgggaa ctgttttagg ttctcgtctc ctaccagaac
      601 cacatatcct gacggggtcg gattccacat cgactccctt cctcaggtcg ggccacaaaa
      661 acggccccca aagtccct
//
