LOCUS       sequence-104295-         796 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Clathrin Vesicles, Excitation: N/A, Emission: N/A.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 796)
  TITLE     Davidson plasmids
REFERENCE   2  (bases 1 to 796)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: Clathrin-15
FEATURES             Location/Qualifiers
     source          1..796
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      28..37
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             34..579
                     /codon_start=1
                     /product="human O6-alkylguanine-DNA-alkyltransferase 
                     variant that forms covalent bonds with benzylguanine 
                     derivatives"
                     /label=SNAP-tag(R)
                     /note="fast-reacting variant, optimized for mammalian 
                     expression"
                     /translation="MDKDCEMKRTTLDSPLGKLELSGCEQGLHRIIFLGKGTSAADAVE
                     VPAPAAVLGGPEPLMQATAWLNAYFHQPEAIEEFPVPALHHPVFQQESFTRQVLWKLLK
                     VVKFGEVISYSHLAALAGNPAATAAVKTALSGNPVPILIPCHRVVQGDLDVGGYEGGLA
                     VKEWLLAHEGHRLGKPGLG"
ORIGIN
        1 ccgtcagatc cgctagcgct accggtcgcc accatggaca aagactgcga aatgaagcgc
       61 accaccctgg atagccctct gggcaagctg gaactgtctg ggtgcgaaca gggcctgcac
      121 cgtatcatct tcctgggcaa aggaacatct gccgccgacg ccgtggaagt gcctgcccca
      181 gccgccgtgc tgggcggacc agagccactg atgcaggcca ccgcctggct caacgcctac
      241 tttcaccagc ctgaggccat cgaggagttc cctgtgccag ccctgcacca cccagtgttc
      301 cagcaggaga gctttacccg ccaggtgctg tggaaactgc tgaaagtggt gaagttcgga
      361 gaggtcatca gctacagcca cctggccgcc ctggccggca atcccgccgc caccgccgcc
      421 gtgaaaaccg ccctgagcgg aaatcccgtg cccattctga tcccctgcca ccgggtggtg
      481 cagggcgacc tggacgtggg gggctacgag ggcgggctcg ccgtgaaaga gtggctgctg
      541 gcccacgagg gccacagact gggcaagcct gggctgggtt ccaaactcag atctcgagct
      601 caagcttcga attctgcagt cgacatggct gatgactttg gcttcttctc gtcgtcggag
      661 agtggtgccc cggaggcggc ggaggaggac ccggcggccg ccttcctggc ccagcaggag
      721 agcgagattg caggcataga gaacgacgag ggcttcgggg cacctgccgg cagccatgcg
      781 gcccccgcac agccgg
//
