LOCUS       sequence-104296-        1023 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  docking site transgenesis plasmid for GoldenGate Cloning and insect 
            transgenesis, YFP-NLS marker.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1023)
  AUTHORS   Volohonsky G, Terenzi O, Soichot J, Naujoks DA, Nolan T, Windbichler
            N, Kapps D, Smidler AL, Vittu A, Costa G, Steinert S, Levashina EA, 
            Blandin SA, Marois E
  TITLE     Tools for Anopheles gambiae Transgenesis.
  JOURNAL   G3 (Bethesda). 2015 Apr 13. pii: g3.115.016808. doi: 
            10.1534/g3.115.016808.
  PUBMED    25869647
REFERENCE   2  (bases 1 to 1023)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "G3 
            (Bethesda). 2015 Apr 13. pii: g3.115.016808. doi: 
            10.1534/g3.115.016808."
COMMENT     Sequence Label: pDSAYN
FEATURES             Location/Qualifiers
     source          1..1023
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     polyA_signal    209..291
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(246..265)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     301..320
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     protein_bind    349..418
                     /label=attB
                     /bound_moiety="phage phi-C31 integrase"
                     /note="attB site for the phi-C31 integrase (Groth et al., 
                     2000)"
     regulatory      933..942
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     complement(984..1005)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
ORIGIN
        1 tctcggtcta ttcttttgat ttataaggga ttttgccgat ttcggcctat tggttaaaaa
       61 atgagctgat ttaacaaaaa tttaacgcgg gtctccgctt ctagacataa tcagccatac
      121 cacatttgta gaggttttac ttgctttaaa aaacctccca cacctccccc tgaacctgaa
      181 acataaaatg aatgcaattg ttgttgttaa cttgtttatt gcagcttata atggttacaa
      241 ataaagcaat agcatcacaa atttcacaaa taaagcattt ttcttcactg cattctagtt
      301 gtggtttgtc caaactcatc aatgtatctc gacgatgtag gtcacagtct cgaagccgcg
      361 gtgcgggtgc cagggcgtgc ccttgggctc cccgggcgcg tactccacct cacccatctg
      421 gtccatcatg atgaacgggt cgaggtggcg gtagttgatc ccggcgaacg cgcggcgcac
      481 cgggaagccc tcgccctcga aaccgctggg cgcggtggtc acggtgagca cgggacgtgc
      541 gacggcgtcg gcgggtgcgg atacgcgggg cagcgtcagc gggttctcga cggtcacggc
      601 gggcaattcc tgcagacttc cggtatctcg cgtttgtttg atcgcacggt tcccacaatg
      661 gttaattcga gctcgcccgg ggatctaatt caattagaga ctaattcaat tagagctaat
      721 tcaattagga tccaagctta tcgatttcga accctcgacc gccggagtat aaatagaggc
      781 gcttcgtcta cggagcgaca attcaattca aacaagcaaa gtgaacacgt cgctaagcga
      841 aagctaagca aataaacaag cgcagctgaa caagctaaac aatcggggta ccgctagagt
      901 cgacggtacc gcgggcccgg gatccaccgg tcgccaccat ggtgagcaag ggcgaggagc
      961 tgttcaccgg ggtggtgccc atcctggtcg agctggacgg cgacgtaaac ggccacaagt
     1021 tca
//
