LOCUS       sequence-104306-        1089 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Riboprobe for in vitro transcription of Plexin A2 (m2).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1089)
  TITLE     Christiana Ruhrberg plasmids
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1089)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: PLEXIN A2 (m2)
FEATURES             Location/Qualifiers
     source          1..1089
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     3..22
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        3..21
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     promoter        complement(774..792)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(775..792)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(810..826)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(810..826)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(823..845)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    834..850
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(858..864,865..882,883..888))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    903..924
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(1041..1058)
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 tgtaatacga ctcactatag ggcgaattgg gccctctaga tgcatgctcg agcggccgcc
       61 agtgtgatgg atatctgcag aattcggctt ccaagtccca ccagcagtat acttttgtga
      121 atccttctgt gctgtcactc agcccgatcc ggggaccaga gtcaggaggt accatggtga
      181 ccatcacagg ccattacctt ggtgctggga gcagtgtggc agtgtacctg ggcaatcaga
      241 cctgtgaatt ctatgggagg tcaatgaatg agattgtatg tgtttcaccc ccatcatcca
      301 atggactagg accagtccct gtctccgtga gtgtcgacag agcccgggtg gatagcagtc
      361 tgcagttcga gtatatagat gacccacggg tccaacgtat tgagccagag tggagtatca
      421 ctagtgggca cacaccccta accatcacag gcttcaactt ggatgtcatt caggagccca
      481 gggtccgagt caaatttaat ggcaaagaat ctgtcaatgt atgcacagtg gtaaacacaa
      541 ccaccctcac ctgtctggca ccctctctga ccagtgacta ccgcccaggt ctggacactg
      601 tggaacggcc agatgagttt ggatttctct ttaacaatgt tcaatcctta ctcatctata
      661 acgacaccaa gttcatctac taccccaacc caacgaagcc gaattccagc acactggcgg
      721 ccgttactag tggatccgag ctcggtacca agcttgatgc atagcttgag tattctatag
      781 tgtcacctaa atagcttggc gtaatcatgg tcatagctgt ttcctgtgtg aaattgttat
      841 ccgctcacaa ttccacacaa catacgagcc ggaagcataa agtgtaaagc ctggggtgcc
      901 taatgagtga gctaactcac attaattgcg ttgcgctcac tgcccgcttt ccagtcggga
      961 aacctgtcgt gccagctgca ttaatgaatc ggccaacgcg cggggagagg cggtttgcgt
     1021 attgggcgct cttccgcttc ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg
     1081 cgagcggta
//
