LOCUS       sequence-104307-        1079 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Riboprobe for in vitro transcription of Plexin A2 (m1).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1079)
  TITLE     Christiana Ruhrberg plasmids
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1079)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: PLEXIN A2 (m1)
FEATURES             Location/Qualifiers
     source          1..1079
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     2..21
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        2..20
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     promoter        complement(801..819)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(802..819)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(837..853)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(837..853)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(850..872)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    861..877
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(885..891,892..909,910..915))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    930..951
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
ORIGIN
        1 gtaatacgac tcactatagg gcgaattggg ccctctagat gcatgctcga gcggccgcca
       61 gtgtgatgga tatctgcaga attcggcttg ggaatctgac tgaggtggag ggacaggtat
      121 ctgggagtca agtcatctgc atctcacctg gacccaagga tgtccctgtc atccctctgg
      181 atcaagactg gtttggccta gagctgcagc tgagatccaa agagacagga aagatctttg
      241 tcagcaccga attcaagttc tataactgca gtgcccacca actgtgcctg tcctgtgtta
      301 acagcgcctt ccgctgccat tggtgcaagt accgtaacct ctgcacacat gaccccacta
      361 cctgttcctt ccaggaaggc aggatcaatg tttcagagga ctgtccccag ctcgtgccca
      421 cggaggagat tctgatccca gttggggaag taaaaccaat cacccttaag gcccgaaacc
      481 tgccccagcc ccagtctggc cagcgaggct acgagtgtgt gctcagcatt caaggggctg
      541 tccaccgggt ccctgccctg cgtttcaaca gttccagtgt gcagtgccaa aacagctcgt
      601 accagtatga tgggatggac atcagcaacc tagcagtgga ctttgctgta gtatggaatg
      661 gcaacttcat tattgacaac cctcaggacc tgaaagtaca tctctacaag tgtgcagccc
      721 agaagccgaa ttccagcaca ctggcggccg ttactagtgg atccgagctc ggtaccaagc
      781 ttgatgcata gcttgagtat tctatagtgt cacctaaata gcttggcgta atcatggtca
      841 tagctgtttc ctgtgtgaaa ttgttatccg ctcacaattc cacacaacat acgagccgga
      901 agcataaagt gtaaagcctg gggtgcctaa tgagtgagct aactcacatt aattgcgttg
      961 cgctcactgc ccgctttcca gtcgggaaac ctgtcgtgcc agctgcatta atgaatcggc
     1021 caacgcgcgg ggagaggcgg tttgcgtatt gggcgctctt ccgcttcctc gctcactga
//
