LOCUS       sequence-104313-         589 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Gateway vector for generating nucleus-targeted fluorescent protein 
            fusions in fungi.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 589)
  AUTHORS   Gong X, Hurtado O, Wang B, Yi M, Giraldo M, Wu C, Valent B, Goodin 
            MM, Farman M
  TITLE     pFPL vectors for high-throughput protein localization in fungi: 
            detecting cytoplasmic accumulation of putative effector proteins.
  JOURNAL   Mol Plant Microbe Interact. 2014 Nov 12.
  PUBMED    25390188
REFERENCE   2  (bases 1 to 589)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Plant 
            Microbe Interact. 2014 Nov 12."
COMMENT     Sequence Label: pFPNL1
FEATURES             Location/Qualifiers
     source          1..589
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    complement(105..229)
                     /gene="mutant version of attR"
                     /label=attR2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(394..412)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
ORIGIN
        1 tgatctcagc caccgcgaaa atgacatcaa aaacgccatt aacctgatgt tctggggaat
       61 ataaatgtca ggctccctta tacacagcca gtctgcaggt cgaccatagt gactggatat
      121 gttgtgtttt acagtattat gtagtctgtt ttttatgcaa aatctaattt aatatattga
      181 tatttatatc attttacgtt tctcgttcag ctttcttgta caaagtggtg atagggcccg
      241 ggatcctgat ggtgagcaag ggcgaggagg ataacatggc catcatcaag gagttcatgc
      301 gcttcaaggt gcacatggag ggctccgtga acggccacga gttcgagatc gagggcgagg
      361 gcgagggccg cccctacgag ggcacccaga ccgccaagct gaaggtgacc aagggtggcc
      421 ccctgccctt cgcctgggac atcctgtccc ctcagttcat gtacggctcc aaggcctacg
      481 tgaagcaccc cgccgacatc cccgactact tgaagctgtc cttccccgag ggcttcaagt
      541 gggagcgcgt gatgaacttc gaggacggcg gcgtggtgac cgtgaccca
//
