LOCUS       sequence-104316-         618 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Gateway vector for generating yellow fluorescent protein fusions in 
            fungi.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 618)
  AUTHORS   Gong X, Hurtado O, Wang B, Yi M, Giraldo M, Wu C, Valent B, Goodin 
            MM, Farman M
  TITLE     pFPL vectors for high-throughput protein localization in fungi: 
            detecting cytoplasmic accumulation of putative effector proteins.
  JOURNAL   Mol Plant Microbe Interact. 2014 Nov 12.
  PUBMED    25390188
REFERENCE   2  (bases 1 to 618)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Plant 
            Microbe Interact. 2014 Nov 12."
COMMENT     Sequence Label: pFPL-Yh2
FEATURES             Location/Qualifiers
     source          1..618
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    complement(95..219)
                     /gene="mutant version of attR"
                     /label=attR2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(272..293)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(533..552)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
ORIGIN
        1 caccgcgaaa atgacatcaa aaacgccatt aacctgatgt tctggggaat ataaatgtca
       61 ggctccctta tacacagcca gtctgcaggt cgaccatagt gactggatat gttgtgtttt
      121 acagtattat gtagtctgtt ttttatgcaa aatctaattt aatatattga tatttatatc
      181 attttacgtt tctcgttccg ctttcttgta caaagtggtg gcagcagcag tgagcaaggg
      241 cgaggagctg ttcaccgggg tggtgcccat cctggtcgag ctggacggcg acgtaaacgg
      301 ccacaagttc agcgtgtccg gcgagggcga gggcgatgcc acctacggca agctgaccct
      361 gaagttcatc tgcaccaccg gcaagctgcc cgtgccctgg cccaccctcg tgaccacctt
      421 cggctacggc ctgcagtgct tcgcccgcta ccccgaccac atgaagcagc acgacttctt
      481 caagtccgcc atgcccgaag gctacgtcca ggagcgcacc atcttcttca aggacgacgg
      541 caactacaag acccgcgccg aggtgaagtt cgagggcgac accctggtga accgcatcga
      601 gctgaagggc atcgactt
//
