LOCUS       sequence-104319-         665 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  HIV-1 integrase F185H/C280S in pET-15b.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 665)
  AUTHORS   Jenkins TM, Engelman A, Ghirlando R, Craigie R
  TITLE     A soluble active mutant of HIV-1 integrase: involvement of both the 
            core and carboxyl-terminal domains in multimerization.
  JOURNAL   J Biol Chem. 1996 Mar 29;271(13):7712-8.
  PUBMED    8631811
REFERENCE   2  (bases 1 to 665)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Biol 
            Chem."; date: "1996-03-29"; volume: "271(13)"; pages: "7712-8"
COMMENT     Sequence Label: HIV-1 integrase F185H/C280S in pET15b
FEATURES             Location/Qualifiers
     source          1..665
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             27..44
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             54..71
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
ORIGIN
        1 gaaggagata taccatgggc agcagccatc atcatcatca tcacagcagc ggcctggtgc
       61 cgcgcggcag ccatatgttt ttagatggaa tagataaggc ccaagaagaa catgagaaat
      121 atcacagtaa ttggagagca atggctagtg attttaacct gccacctgta gtagcaaaag
      181 aaatagtagc cagctgtgat aaatgtcagc taaaagggga agccatgcat ggacaagtag
      241 actgtagccc aggaatatgg cagctagatt gtacacattt agaaggaaaa gttatcttgg
      301 tagcagttca tgtagccagt ggatatatag aagcagaagt aattccagca gagacagggc
      361 aagaaacagc atacttcctc ttaaaattag caggaagatg gccagtaaaa acagtacata
      421 cagacaatgg cagcaatttc accagtacta cagttaaggc cgcctgttgg tgggcgggga
      481 tcaagcagga atttggcatt ccctacaatc cccaaagtca gggggtaata gaatctatga
      541 ataaagaatt aaagaaaatt ataggacagg taagagatca ggctgaacat cttaagacag
      601 cagtacaaat ggcagtattc atccacaatc ataaaagaaa aggggggatt ggggggtaca
      661 gtgca
//
