LOCUS       sequence-104321-         662 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  HIV-1 integrase W235F in pET-15b.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 662)
  AUTHORS   Li M, Mizuuchi M, Burke TR Jr, Craigie R
  TITLE     Retroviral DNA integration: reaction pathway and critical 
            intermediates.
  JOURNAL   EMBO J. 2006 Mar 22;25(6):1295-304. Epub 2006 Feb 16.
  PUBMED    16482214
REFERENCE   2  (bases 1 to 662)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "EMBO J."; 
            date: "2006-03-22"; volume: "25(6)"; pages: "1295-304. Epub 2006 Feb
            16"
COMMENT     Sequence Label: HIV-1 integrase W235F in pET-15b
FEATURES             Location/Qualifiers
     source          1..662
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             24..41
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             51..68
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
ORIGIN
        1 ggagatatac catgggcagc agccatcatc atcatcatca cagcagcggc ctggtgccgc
       61 gcggcagcca tatgttttta gatggaatag ataaggccca agaagaacat gagaaatatc
      121 acagtaattg gagagcaatg gctagtgatt ttaacctgcc acctgtagta gcaaaagaaa
      181 tagtagccag ctgtgataaa tgtcagctaa aaggggaagc catgcatgga caagtagact
      241 gtagcccagg aatatggcag ctagattgta cacatttaga aggaaaagtt atcttggtag
      301 cagttcatgt agccagtgga tatatagaag cagaagtaat tccagcagag acagggcaag
      361 aaacagcata cttcctctta aaattagcag gaagatggcc agtaaaaaca gtacatacag
      421 acaatggcag caatttcacc agtactacag ttaaggccgc ctgttggtgg gcggggatca
      481 agcaggaatt tggcattccc tacaatcccc aaagtcaggg ggtaatagaa tctatgaata
      541 aagaattaaa gaaaattata ggacaggtaa gagatcaggc tgaacatctt aagacagcag
      601 tacaaatggc agtattcatc cacaatttta aaagaaaagg ggggattggg gggtacagtg
      661 ca
//
