LOCUS       sequence-104334-         668 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Mouse NLRC4 expressed under the constitutive, moderate-level 
            retroviral LTR promoter, expresses in mammalian cells; IRES-GFP 
            reporter downstream of NLRC4.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 668)
  AUTHORS   Kofoed EM, Vance RE
  TITLE     Innate immune recognition of bacterial ligands by NAIPs determines 
            inflammasome specificity.
  JOURNAL   Nature. 2011 Aug 28;477(7366):592-5. doi: 10.1038/nature10394.
  PUBMED    21874021
REFERENCE   2  (bases 1 to 668)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10"; journalName: 
            "Nature"; date: "2011-08-28- 28"; volume: "477"; issue: "7366"; 
            pages: "592-5"
COMMENT     Sequence Label: mscv2.2-NLRC4
FEATURES             Location/Qualifiers
     source          1..668
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     misc_feature    14..566
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     misc_feature    16..582
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(176..195)
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     primer_bind     405..422
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
ORIGIN
        1 atggttgtgg ccatattatc atcgtgtttt tcaaaggaaa accacgtccc cgtggttcgg
       61 ggggcctaga cgttttttta acctcgacta aacacatgta aagcatgtgc accgaggccc
      121 cagatcagat cccatacaat ggggtacctt ctgggcatcc ttcagcccct tgttgaatac
      181 gcttgaggag agccatttga ctctttccac aactatccaa ctcacaacgt ggcactgggg
      241 ttgtgccgcc tttgcaggtg tatcttatac acgtggcttt tggccgcaga ggcacctgtc
      301 gccaggtggg gggttccgct gcctgcaaag ggtcgctaca gacgttgttt gtcttcaaga
      361 agcttccaga ggaactgctt ccttcacgac attcaacaga ccttgcattc ctttggcgag
      421 aggggaaaga cccctaggaa tgctcgtcaa gaagacaggg ccaggtttcc gggccctcac
      481 attgccaaaa gacggcaata tggtggaaaa taacatatag acaaacgcac accggcctta
      541 ttccaagcgg cttcggccag taacgttagg ggggggggcg gaattcgata tcaagcttat
      601 cgataccgtc gacgtttaaa cgcggccgct taagcagtca ctagtttaaa ggtgccttta
      661 atagcgct
//
