LOCUS       sequence-104336-         711 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  6xMYC-tagged full-length FlaA from Legionella pneumophila, expressed
            under the constitutive, moderate-level retroviral LTR promoter for 
            mammalian cell expression; IRES-GFP reporter downstream of FlaA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 711)
  AUTHORS   Kofoed EM, Vance RE
  TITLE     Innate immune recognition of bacterial ligands by NAIPs determines 
            inflammasome specificity.
  JOURNAL   Nature. 2011 Aug 28;477(7366):592-5. doi: 10.1038/nature10394.
  PUBMED    21874021
REFERENCE   2  (bases 1 to 711)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10"; journalName: 
            "Nature"; date: "2011-08-28- 28"; volume: "477"; issue: "7366"; 
            pages: "592-5"
COMMENT     Sequence Label: mscv2.2-6mycFlaA
FEATURES             Location/Qualifiers
     source          1..711
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(575..592)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
ORIGIN
        1 gatactatcg cgattggtgg tactgttgct aacattggtt taagtgccaa tatctctaaa
       61 gacacagtag ggatagattc actggatgtg agcacagcgt ctggcgctca aacagcgatt
      121 aagagaattg atgcagcact caatagcgta aactctaatc gggctaatat gggtgctctg
      181 caaaatcgct ttgaatcaac gatagccaac ctgcaaaacg tatcagacaa cttatcagct
      241 gctagaagtc gtattcaaga tgctgattac gctgctgaaa tggctagttt gaccaaaaac
      301 caaattctgc aacaagcagg tacagcgatg ttggcacaag ctaatagcct accgcaatct
      361 gtattatctt tgttaggtcg atagtcgacg gtatcgataa gcttgatatc gaattccgcc
      421 ccccccccta acgttactgg ccgaagccgc ttggaataag gccggtgtgc gtttgtctat
      481 atgttatttt ccaccatatt gccgtctttt ggcaatgtga gggcccggaa acctggccct
      541 gtcttcttga cgagcattcc taggggtctt tcccctctcg ccaaaggaat gcaaggtctg
      601 ttgaatgtcg tgaaggaagc agttcctctg gaagcttctt gaagacaaac aacgtctgta
      661 gcgacccttt gcaggcagcg gaacccccca cctggcgaca ggtgcctctg c
//
