LOCUS       sequence-104354-         955 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses PP7 coat protein fused to mCherry.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 955)
  AUTHORS   Coulon A, Ferguson ML, de Turris V, Palangat M, Chow CC, Larson DR
  TITLE     Kinetic competition during the transcription cycle results in 
            stochastic RNA processing.
  JOURNAL   Elife. 2014 Oct 1;3. doi: 10.7554/eLife.03939.
  PUBMED    25271374
REFERENCE   2  (bases 1 to 955)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Elife."; 
            date: "2014-10-1"; volume: "3. doi"; pages: " 10.7554/eLife.03939"
COMMENT     Sequence Label: PP7_mCherry
FEATURES             Location/Qualifiers
     source          1..955
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(113..129)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(114..130)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     protein_bind    197..217
                     /gene="mutant version of attB"
                     /label=attB3
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     CDS             complement(219..926)
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="VSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEGT
                     QTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNFE
                     DGGVVTVTQDSSLQDGEFIYKVKLRGTNFPYDGPVMQKKTMGWEASSERMYPEDGALKG
                     EIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERAE
                     GRHSTGGMDELYK"
     primer_bind     complement(497..516)
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     766..784
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     protein_bind    928..952
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 gtcaatcttt cacaaatttt gtaatccaga ggttgattat cgataagctt gatatcgaat
       61 tgtacctagt ggaaccggaa cccttaaaca tgatctcgtg aagcgagctt atcgataccg
      121 tcgacctcga tgctttgcat acttctgcct gctggggagc ctggggactt tccacaccct
      181 agatcccttg aattatcaac tatgtataat aaagttgatt acttgtacag ctcgtccatg
      241 ccgccggtgg agtggcggcc ctcggcgcgt tcgtactgtt ccacgatggt gtagtcctcg
      301 ttgtgggagg tgatgtccaa cttgatgttg acgttgtagg cgccgggcag ctgcacgggc
      361 ttcttggcct tgtaggtggt cttgacctca gcgtcgtagt ggcctccgtc cttcagcttc
      421 agcctctgct tgatctcgcc cttcagggcg ccgtcctcgg ggtacatccg ctcggaggag
      481 gcctcccagc ccatggtctt cttctgcatt acggggccgt cgtaggggaa gttggtgccg
      541 cgcagcttca ccttgtagat gaactcgccg tcctgcaggg aggagtcctg ggtcacggtc
      601 accacgccgc cgtcctcgaa gttcatcacg cgctcccact tgaagccctc ggggaaggac
      661 agcttcaagt agtcggggat gtcggcgggg tgcttcacgt aggccttgga gccgtacatg
      721 aactgagggg acaggatgtc ccaggcgaag ggcagggggc cacccttggt caccttcagc
      781 ttggcggtct gggtgccctc gtaggggcgg ccctcgccct cgccctcgat ctcgaactcg
      841 tggccgttca cggagccctc catgtgcacc ttgaagcgca tgaactcctt gatgatggcc
      901 atgttatcct cctcgccctt gctcacgccc actttgtaca agaaagttga agaac
//
