LOCUS       sequence-104357-         811 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Contains a truncated Arabidopsis beta-carotene hydroxylase cDNA, the
            product of which converts beta-carotene primarily to monocyclic 
            beta-cryptoxanthin in E. coli. Use with pAC-BETA or pAC-BETAipi. .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 811)
  AUTHORS   Cunningham FX Jr, Gantt E
  TITLE     A study in scarlet: enzymes of ketocarotenoid biosynthesis in the 
            flowers of Adonis aestivalis.
  JOURNAL   Plant J. 2005 Feb;41(3):478-92.
  PUBMED    15659105
REFERENCE   2  (bases 1 to 811)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant J."; 
            date: "2005-02"; volume: "41(3)"; pages: "478-92"
COMMENT     Sequence Label: pAtCHYb1-Delta-129N
FEATURES             Location/Qualifiers
     source          1..811
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             3..20
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             24..56
                     /codon_start=1
                     /product="leader peptide from bacteriophage T7 gene 10"
                     /label=T7 tag (gene 10 leader)
                     /note="promotes efficient translation in E. coli"
                     /translation="MASMTGGQQMG"
     CDS             join(60..68,69..83)
                     /codon_start=1
                     /product="Xpress(TM) epitope tag, including an enterokinase
                     recognition and cleavage site"
                     /label=Xpress(TM) tag
                     /translation="DLYDDDDK"
ORIGIN
        1 ctcatcatca tcatcatcat ggtatggcta gcatgactgg tggacagcaa atgggtcggg
       61 atctgtacga cgatgacgat aaggatcgat ggggatctca atgttggaaa tgtttggtac
      121 atttgctctc tctgttggtg ctgctgttgg tatggaattc tgggcaagat gggctcatag
      181 agctctgtgg cacgcttctc tatggaatat gcatgagtca catcacaaac caagagaagg
      241 accgtttgag ctaaacgatg tttttgctat agtgaacgct ggtccagcga ttggtctcct
      301 ctcttatgga ttcttcaata aaggactcgt tcctggtctc tgctttggcg ccgggttagg
      361 cataacggtg tttggaatcg cctacatgtt tgtccacgat ggtctcgtgc acaagcgttt
      421 ccctgtaggt cccatcgccg acgtccctta cctccgaaag gtcgccgccg ctcaccagct
      481 acatcacaca gacaagttca atggtgtacc atatggactg tttcttggac ccaaggaatt
      541 ggaagaagtt ggaggaaatg aagagttaga taaggagatt agtcggagaa tcaaatcata
      601 caaaaaggcc tcgggctccg ggtcgagttc gagttcttga ctttaaacaa gttttaaatc
      661 ccaaattctt tttttgtctt ctgtcattat gatcatctta agacggtctg cggccgatcc
      721 gagctcgaga tctgcagctg gtaccatatg ggaattcgaa gcttggctgt tttggcggat
      781 gagagaagat tttcagcctg atacagatta a
//
