LOCUS       sequence-104363-         396 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  MuLE (Multiple Lentiviral Expression) Entry vector containing a 7SK 
            RNA polymerase III promoter for shRNA expression. Compatible with 
            MultiSite Gateway cloning.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 396)
  AUTHORS   Albers J, Danzer C, Rechsteiner M, Lehmann H, Brandt LP, Hejhal T, 
            Catalano A, Busenhart P, Goncalves AF, Brandt S, Bode PK, 
            Bode-Lesniewska B, Wild PJ, Frew IJ
  TITLE     A versatile modular vector system for rapid combinatorial mammalian 
            genetics.
  JOURNAL   J Clin Invest. 2015 Mar 9. pii: 79743. doi: 10.1172/JCI79743.
  PUBMED    25751063
REFERENCE   2  (bases 1 to 396)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Clin 
            Invest. 2015 Mar 9. pii: 79743. doi: 10.1172/JCI79743."
COMMENT     Sequence Label: pMuLE ENTR 7SK R4-R3
FEATURES             Location/Qualifiers
     source          1..396
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    complement(12..135)
                     /gene="mutant version of attR"
                     /label=attR4
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
ORIGIN
        1 agtagttgat tcatagtgac tggatatgtt gtgttttaca gtattatgta gtctgttttt
       61 tatgcaaaat ctaatttaat atattgatat ttatatcatt ttacgtttct cgttcaactt
      121 ttctatacaa agttgctatc gatctagtgc tgcagtattt agcatgcccc acccatctgc
      181 aaggcattct ggatagtgtc aaaacagccg gaaatcaagt ccgtttatct caaactttag
      241 cattttggga ataaatgata tttgctatgc tggttaaatt agattttagt taaatttcct
      301 gctgaagctc tagtacgata agtaacttga cctaagtgta aagttgagat ttccttcagg
      361 tttatatagc ttgtgcgccg cctgggtacc tcggtc
//
