LOCUS       sequence-104366-         600 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  MuLE (Multiple Lentiviral Expression) Entry vector containing a H1 
            promoter for shRNA expression. Compatible with MultiSite Gateway 
            cloning.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 600)
  AUTHORS   Albers J, Danzer C, Rechsteiner M, Lehmann H, Brandt LP, Hejhal T, 
            Catalano A, Busenhart P, Goncalves AF, Brandt S, Bode PK, 
            Bode-Lesniewska B, Wild PJ, Frew IJ
  TITLE     A versatile modular vector system for rapid combinatorial mammalian 
            genetics.
  JOURNAL   J Clin Invest. 2015 Mar 9. pii: 79743. doi: 10.1172/JCI79743.
  PUBMED    25751063
REFERENCE   2  (bases 1 to 600)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Clin 
            Invest. 2015 Mar 9. pii: 79743. doi: 10.1172/JCI79743."
COMMENT     Sequence Label: pMuLE ENTR H1 L5-L2
FEATURES             Location/Qualifiers
     source          1..600
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    58..78
                     /gene="mutant version of attB"
                     /label=attB5
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     promoter        126..340
                     /label=H1 promoter
                     /note="human H1 RNA promoter"
     primer_bind     261..280
                     /label=H1
                     /note="Human H1 promoter, forward primer"
     protein_bind    complement(570..594)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 gactgatagt gacctgttcg ttgcaacaaa ttgatgagca atgctttttt ataatgccaa
       61 ctttgtatac aaaagttgct atcgatccaa ttcagtcgac tggatccccc gggctgcagg
      121 aattcgaacg ctgacgtcat caacccgctc caaggaatcg cgggcccagt gtcactaggc
      181 gggaacaccc agcgcgcgtg cgccctggca ggaagatggc tgtgagggac aggggagtgg
      241 cgccctgcaa tatttgcatg tcgctatgtg ttctgggaaa tcaccataaa cgtgaaatgt
      301 ctttggattt gggaatctta taagttctgt atgagaccac agatctcggc gttgtgcatg
      361 tcgggcgact gctccatgat cttgcgccgc tcgatctgcg accacaccat aaaggcgttc
      421 atgggccgct tgatgtggcc actgggcgtc ttgcaccagc tggggtcgtc cgccttgccg
      481 cccgtcgacg cggtggagcc aggcgtcggg gaggacgcga tgcccagctc caggccggcg
      541 cccgagtccg agctcttttt tgaattcgta cccagctttc ttgtacaaag ttggcattat
//
