LOCUS       sequence-104368-         694 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  MuLE (Multiple Lentiviral Expression) Entry vector containing a CMV 
            promoter and multiple cloning site module. Compatible with MultiSite
            Gateway cloning.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 694)
  AUTHORS   Albers J, Danzer C, Rechsteiner M, Lehmann H, Brandt LP, Hejhal T, 
            Catalano A, Busenhart P, Goncalves AF, Brandt S, Bode PK, 
            Bode-Lesniewska B, Wild PJ, Frew IJ
  TITLE     A versatile modular vector system for rapid combinatorial mammalian 
            genetics.
  JOURNAL   J Clin Invest. 2015 Mar 9. pii: 79743. doi: 10.1172/JCI79743.
  PUBMED    25751063
REFERENCE   2  (bases 1 to 694)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Clin 
            Invest. 2015 Mar 9. pii: 79743. doi: 10.1172/JCI79743."
COMMENT     Sequence Label: pMuLE ENTR CMV R4-R3
FEATURES             Location/Qualifiers
     source          1..694
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        156..359
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     310..330
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     404..423
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        404..422
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    533..656
                     /gene="mutant version of attR"
                     /label=attR3
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
ORIGIN
        1 acttggcagt acatcaagtg tatcatatgc caagtacgcc ccctattgac gtcaatgacg
       61 gtaaatggcc cgcctggcat tatgcccagt acatgacctt atgggacttt cctacttggc
      121 agtacatcta cgtattagtc atcgctatta ccatggtgat gcggttttgg cagtacatca
      181 atgggcgtgg atagcggttt gactcacggg gatttccaag tctccacccc attgacgtca
      241 atgggagttt gttttggcac caaaatcaac gggactttcc aaaatgtcgt aacaactccg
      301 ccccattgac gcaaatgggc ggtaggcgtg tacggtggga ggtctatata agcagagctc
      361 tctggctaac tagagaaccc actgcttact ggcttatcga aattaatacg actcactata
      421 gggagaccca agcttggtac cgagctcgga tccactagta acggccgcca gtgtgctgga
      481 attctgcaga tatccatcac actggcggcc gctcgagcat gcatctagag gacaactttg
      541 tataataaag ttgaacgaga aacgtaaaat gatataaata tcaatatatt aaattagatt
      601 ttgcataaaa aacagactac ataatactgt aaaacacaac atatccagtc actatgaatc
      661 aactacttag atggtattag tgacctgtac tgca
//
