LOCUS       sequence-104372-         582 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  MuLE (Multiple Lentiviral Expression) Entry vector containing a H1 
            promoter for shRNA expression. Compatible with MultiSite Gateway 
            cloning.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 582)
  AUTHORS   Albers J, Danzer C, Rechsteiner M, Lehmann H, Brandt LP, Hejhal T, 
            Catalano A, Busenhart P, Goncalves AF, Brandt S, Bode PK, 
            Bode-Lesniewska B, Wild PJ, Frew IJ
  TITLE     A versatile modular vector system for rapid combinatorial mammalian 
            genetics.
  JOURNAL   J Clin Invest. 2015 Mar 9. pii: 79743. doi: 10.1172/JCI79743.
  PUBMED    25751063
REFERENCE   2  (bases 1 to 582)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Clin 
            Invest. 2015 Mar 9. pii: 79743. doi: 10.1172/JCI79743."
COMMENT     Sequence Label: pMuLE ENTR H1 R4-R3
FEATURES             Location/Qualifiers
     source          1..582
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    complement(21..144)
                     /gene="mutant version of attR"
                     /label=attR4
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        192..406
                     /label=H1 promoter
                     /note="human H1 RNA promoter"
     primer_bind     327..346
                     /label=H1
                     /note="Human H1 promoter, forward primer"
ORIGIN
        1 taccatctaa gtagttgatt catagtgact ggatatgttg tgttttacag tattatgtag
       61 tctgtttttt atgcaaaatc taatttaata tattgatatt tatatcattt tacgtttctc
      121 gttcaacttt tctatacaaa gttgctatcg atccaattca gtcgactgga tcccccgggc
      181 tgcaggaatt cgaacgctga cgtcatcaac ccgctccaag gaatcgcggg cccagtgtca
      241 ctaggcggga acacccagcg cgcgtgcgcc ctggcaggaa gatggctgtg agggacaggg
      301 gagtggcgcc ctgcaatatt tgcatgtcgc tatgtgttct gggaaatcac cataaacgtg
      361 aaatgtcttt ggatttggga atcttataag ttctgtatga gaccacagat ctcggcgttg
      421 tgcatgtcgg gcgactgctc catgatcttg cgccgctcga tctgcgacca caccataaag
      481 gcgttcatgg gccgcttgat gtggccactg ggcgtcttgc accagctggg gtcgtccgcc
      541 ttgccgcccg tcgacgcggt ggagccaggc gtcggggagg ac
//
