LOCUS       sequence-104374-         554 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  MuLE (Multiple Lentiviral Expression) Entry vector containing a H1 
            promoter for shRNA expression. Compatible with MultiSite Gateway 
            cloning.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 554)
  AUTHORS   Albers J, Danzer C, Rechsteiner M, Lehmann H, Brandt LP, Hejhal T, 
            Catalano A, Busenhart P, Goncalves AF, Brandt S, Bode PK, 
            Bode-Lesniewska B, Wild PJ, Frew IJ
  TITLE     A versatile modular vector system for rapid combinatorial mammalian 
            genetics.
  JOURNAL   J Clin Invest. 2015 Mar 9. pii: 79743. doi: 10.1172/JCI79743.
  PUBMED    25751063
REFERENCE   2  (bases 1 to 554)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Clin 
            Invest. 2015 Mar 9. pii: 79743. doi: 10.1172/JCI79743."
COMMENT     Sequence Label: pMuLE ENTR H1 R4-R3
FEATURES             Location/Qualifiers
     source          1..554
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    complement(39..162)
                     /gene="mutant version of attR"
                     /label=attR3
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        complement(392..482)
                     /label=H1 promoter
                     /note="human H1 RNA promoter"
     primer_bind     complement(452..471)
                     /label=H1
                     /note="Human H1 promoter, forward primer"
ORIGIN
        1 tgcagtacag gtcactaata ccatctaagt agttgattca tagtgactgg atatgttgtg
       61 ttttacagta ttatgtagtc tgttttttat gcaaaatcta atttaatata ttgatattta
      121 tatcatttta cgtttctcgt tcaactttat tatacaaagt tgtcgaattc aaaaaagagc
      181 tcggactcgg gcgccggcct ggagctgggc atcgcgtcct ccccgacgcc tggctccacc
      241 gcgtcgacgg gcggcaaggc ggacgacccc agctggtgca agacgcccag tggccacatc
      301 aagcggccca tgaacgcctt tatggtgtgg tcgcagatcg agcggcgcaa gatcatggag
      361 cagtcgcccg acatgcacaa cgccgagatc tgtggtctca tacagaactt ataagattcc
      421 caaatccaaa gacatttcac gtttatggtg atttcccaga acacatagcg acatgcaaat
      481 attgcagggc gccactcccc tgtccctcac agccatcttc ctgccagggc gcacgcgcgc
      541 tgggtgttcc cgcc
//
