LOCUS       sequence-104397-         621 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  MuLE (Multiple Lentiviral Expression) Entry vector containing a H1 
            promoter for shRNA expression. Compatible with MultiSite Gateway 
            cloning.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 621)
  AUTHORS   Albers J, Danzer C, Rechsteiner M, Lehmann H, Brandt LP, Hejhal T, 
            Catalano A, Busenhart P, Goncalves AF, Brandt S, Bode PK, 
            Bode-Lesniewska B, Wild PJ, Frew IJ
  TITLE     A versatile modular vector system for rapid combinatorial mammalian 
            genetics.
  JOURNAL   J Clin Invest. 2015 Mar 9. pii: 79743. doi: 10.1172/JCI79743.
  PUBMED    25751063
REFERENCE   2  (bases 1 to 621)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Clin 
            Invest. 2015 Mar 9. pii: 79743. doi: 10.1172/JCI79743."
COMMENT     Sequence Label: pMuLE ENTR H1 L1-L4
FEATURES             Location/Qualifiers
     source          1..621
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    57..81
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     promoter        129..343
                     /label=H1 promoter
                     /note="human H1 RNA promoter"
     primer_bind     264..283
                     /label=H1
                     /note="Human H1 promoter, forward primer"
     protein_bind    complement(576..596)
                     /gene="mutant version of attB"
                     /label=attB4
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
ORIGIN
        1 gactgatagt gacctgttcg ttgcaacaaa ttgatgagca atgctttttt ataatgccaa
       61 ctttgtacaa aaaagcaggc tctatcgatc caattcagtc gactggatcc cccgggctgc
      121 aggaattcga acgctgacgt catcaacccg ctccaaggaa tcgcgggccc agtgtcacta
      181 ggcgggaaca cccagcgcgc gtgcgccctg gcaggaagat ggctgtgagg gacaggggag
      241 tggcgccctg caatatttgc atgtcgctat gtgttctggg aaatcaccat aaacgtgaaa
      301 tgtctttgga tttgggaatc ttataagttc tgtatgagac cacagatctc ggcgttgtgc
      361 atgtcgggcg actgctccat gatcttgcgc cgctcgatct gcgaccacac cataaaggcg
      421 ttcatgggcc gcttgatgtg gccactgggc gtcttgcacc agctggggtc gtccgccttg
      481 ccgcccgtcg acgcggtgga gccaggcgtc ggggaggacg cgatgcccag ctccaggccg
      541 gcgcccgagt ccgagctctt ttttgaattc gcacccaact tttctataca aagttggcat
      601 tataagaaag cattgcttat c
//
