LOCUS       sequence-104398-         508 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  MuLE (Multiple Lentiviral Expression) Entry vector containing a H1 
            promoter for shRNA expression. Compatible with MultiSite Gateway 
            cloning.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 508)
  AUTHORS   Albers J, Danzer C, Rechsteiner M, Lehmann H, Brandt LP, Hejhal T, 
            Catalano A, Busenhart P, Goncalves AF, Brandt S, Bode PK, 
            Bode-Lesniewska B, Wild PJ, Frew IJ
  TITLE     A versatile modular vector system for rapid combinatorial mammalian 
            genetics.
  JOURNAL   J Clin Invest. 2015 Mar 9. pii: 79743. doi: 10.1172/JCI79743.
  PUBMED    25751063
REFERENCE   2  (bases 1 to 508)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Clin 
            Invest. 2015 Mar 9. pii: 79743. doi: 10.1172/JCI79743."
COMMENT     Sequence Label: pMuLE ENTR H1 L1-L4
FEATURES             Location/Qualifiers
     source          1..508
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    73..93
                     /gene="mutant version of attB"
                     /label=attB4
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     promoter        complement(326..416)
                     /label=H1 promoter
                     /note="human H1 RNA promoter"
     primer_bind     complement(386..405)
                     /label=H1
                     /note="Human H1 promoter, forward primer"
ORIGIN
        1 taatgatttt attttgactg atagtgacct gttcgttgca acaaattgat aagcaatgct
       61 ttcttataat gccaactttg tatagaaaag ttgggtgcga attcaaaaaa gagctcggac
      121 tcgggcgccg gcctggagct gggcatcgcg tcctccccga cgcctggctc caccgcgtcg
      181 acgggcggca aggcggacga ccccagctgg tgcaagacgc ccagtggcca catcaagcgg
      241 cccatgaacg cctttatggt gtggtcgcag atcgagcggc gcaagatcat ggagcagtcg
      301 cccgacatgc acaacgccga gatctgtggt ctcatacaga acttataaga ttcccaaatc
      361 caaagacatt tcacgtttat ggtgatttcc cagaacacat agcgacatgc aaatattgca
      421 gggcgccact cccctgtccc tcacagccat cttcctgcca gggcgcacgc gcgctgggtg
      481 ttcccgccta gtgacactgg gcccgcga
//
