LOCUS       sequence-104399-         916 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Positive control duet plasmid for SepOTS-dependent split mCherry 
            assembly assay (i.e., N-mCherry is fused to the peptide ME(pS)VD (pS
            = phosphoserine); C-mCherry is fused to TRAP(RK)).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 916)
  AUTHORS   Sawyer N, Gassaway BM, Haimovich AD, Isaacs FJ, Rinehart J, Regan L
  TITLE     Designed Phosphoprotein Recognition in Escherichia coli.
  JOURNAL   ACS Chem Biol. 2014 Nov 21;9(11):2502-7. doi: 10.1021/cb500658w. 
            Epub 2014 Oct 6.
  PUBMED    25272187
REFERENCE   2  (bases 1 to 916)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1021/cb500658w";
            journalName: "ACS Chem Biol"; date: "2014-11-21- 21"; volume: "9"; 
            issue: "11"; pages: "2502-7"
COMMENT     Sequence Label: pNAS1B split mCherry N-ME(pS)VD/TRAP(RK)-C
FEATURES             Location/Qualifiers
     source          1..916
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        join(104..122,123..128,129..145,146..151,152..177)
                     /label=PLtetO-1 promoter
                     /note="modified phage lambda PL promoter with tet operator 
                     sites (Lutz and Bujard, 1997)"
     protein_bind    104..122
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    129..147
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     RBS             187..198
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
     primer_bind     complement(356..374)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     624..643
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     terminator      766..852
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
ORIGIN
        1 aaacttcatt tttaatttaa aaggatctag gtgaagatcc tttttgataa tctcatgacc
       61 aaaatccctt aacgtgagtt ttcgttccac tgagcgaaga tcttccctat cagtgataga
      121 gattgacatc cctatcagtg atagagatac tgagcacatc agcaggacgc actgaccgaa
      181 ttcattaaag aggagaaagg taccatggca tccgtgagca agggcgagga ggataacatg
      241 gccatcatca aggagttcat gcgcttcaag gtgcacatgg agggctccgt gaacggccac
      301 gagttcgaga tcgagggcga gggcgagggc cgcccctacg agggcaccca gaccgccaag
      361 ctgaaggtga ccaagggtgg ccccctgccc ttcgcctggg acatcctgtc ccctcagttc
      421 atgtacggct ccaaggccta cgtgaagcac cccgccgaca tccccgacta cttgaagctg
      481 tccttccccg agggcttcaa gtgggagcgc gtgatgaact tcgaggacgg cggcgtggtg
      541 accgtgaccc aggactcctc cctgcaggac ggcgagttca tctacaaggt gaagctgcgc
      601 ggcaccaact tcccctccga cggccccgta atgcagaaga agaccatggg ctgggaggcc
      661 tcctccgagc ggatgtaccc cgaggacggt ggctctggct ctgggtcgac tggtatggaa
      721 taggtggatt aatgaaagct tcatggtacg cgtgctgcca ggcatcaaat aaaacgaaag
      781 gctcagtcga aagactgggc ctttcgtttt atctgttgtt tgtcggtgaa cgctctcctg
      841 agtaggacaa atccgccgcc ctcctaggat cagcgctagc ggagtgtata ctggcttact
      901 atgttggcac tgatga
//
