LOCUS       sequence-104404-        1092 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Dual Color betaGlobin reporter to study splicing kinetics in vivo.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1092)
  AUTHORS   Coulon A, Ferguson ML, de Turris V, Palangat M, Chow CC, Larson DR
  TITLE     Kinetic competition during the transcription cycle results in 
            stochastic RNA processing.
  JOURNAL   Elife. 2014 Oct 1;3. doi: 10.7554/eLife.03939.
  PUBMED    25271374
REFERENCE   2  (bases 1 to 1092)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Elife."; 
            date: "2014-10-1"; volume: "3. doi"; pages: " 10.7554/eLife.03939"
COMMENT     Sequence Label: CFP_ betaGlobin_24XPP7_24XMS2_Splicing_ reporter
FEATURES             Location/Qualifiers
     source          1..1092
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        3..21
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     3..20
                     /label=SP6
                     /note="SP6 promoter, forward primer"
ORIGIN
        1 gcatttaggt gacactatag aataggatct gatgaaccct ggaatactgg agcgacgcgt
       61 gatcgtcgtc gtttgaagat tcgacctgga ggcacggctg atgctcgtgc tttcttggca
      121 ataagtaccg taggatctga tgaaccctgg aatactggag cgacgcgtga tcgtcgtcgt
      181 ttgaagattc gacctggagg cacggctgat gctcgtgctt tcttggcaat aagtaccgta
      241 ggatctgatg aaccctggaa tactggagcg acgcgtgatc gtcgtcgttt gaagattcga
      301 cctggaggca cggctgatgc tcgtgctttc ttggcaataa gtaccgtagg atctgatgaa
      361 ccctggaata ctggagcgac gcgtgatcgt cgtcgtttga agattcgacc tggaggcacg
      421 gctgatgctc gtgctttctt ggcaataagt accgtaggat ctgatgaacc ctggaatact
      481 ggagcgacgc gtgatcgtcg tcgtttgaag attcgacctg gaggcacggc tgatgctcgt
      541 gctttcttgg caataagtac cgtaggatct gatgaaccct ggaatactgg agcgacgcgt
      601 gatcgtcgtc gtttgaagat tcgacctgga ggcacggctg atgctcgtgc tttcttggca
      661 ataagtaccg taggatctga tgaaccctgg aatactggag cgacgcgtga tcgtcgtcgt
      721 ttgaagattc gacctggagg cacggctgat gctcgtgctt tcttggcaat aagtaccgta
      781 ggatctgatg aaccctggaa tactggagcg acgcgtgatc gtcgtcgttt gaagattcga
      841 cctggaggca cggctgatgc tcgtgctttc ttggcaataa gtaccgtagg atctgatgaa
      901 ccctggaata ctggagcgac gcgtgatcgt cgtcgtttga agattcgacc tggaggcacg
      961 gctgatgctc gtgctttctt ggcaataagt accgtaggat ctgatgaacc ctggaatact
     1021 ggagcgacgc gtgatcgtcg tcgtttgaag attcgacctg gaggcacggc tgatgctcgt
     1081 gctttcttgg ca
//
