LOCUS       sequence-104410-        1012 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Dual Color betaGlobin reporter to study splicing kinetics in vivo.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1012)
  AUTHORS   Coulon A, Ferguson ML, de Turris V, Palangat M, Chow CC, Larson DR
  TITLE     Kinetic competition during the transcription cycle results in 
            stochastic RNA processing.
  JOURNAL   Elife. 2014 Oct 1;3. doi: 10.7554/eLife.03939.
  PUBMED    25271374
REFERENCE   2  (bases 1 to 1012)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Elife."; 
            date: "2014-10-1"; volume: "3. doi"; pages: " 10.7554/eLife.03939"
COMMENT     Sequence Label: CFP_ betaGlobin_24XPP7_24XMS2_Splicing_ reporter
FEATURES             Location/Qualifiers
     source          1..1012
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(join(121..633,634..636,637..639))
                     /codon_start=1
                     /product="N-terminal fragment of mVenus for use in 
                     bimolecular fluorescence complementation (BiFC) (Kodama and
                     Hu, 2010)"
                     /label=VN173
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTWGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYISHNVYITADKQKNGIK
                     ANFKIRHNIE"
     primer_bind     314..333
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     573..594
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
ORIGIN
        1 gttggggtct ttgctcaggg cggactgggt gctcaggtag tggttgtcgg gcagcagcac
       61 ggggccgtcg ccgatggggg tgttctgctg gtagtggtcg gcgagctgca cgctgccgtc
      121 ctcgatgttg tggcggatct tgaagttggc cttgatgccg ttcttctgct tgtcggcggt
      181 gatatagacg ttgtggctga tgtagttgta ctccagcttg tgccccagga tgttgccgtc
      241 ctccttgaag tcgatgccct tcagctcgat gcggttcacc agggtgtcgc cctcgaactt
      301 cacctcggcg cgggtcttgt agttgccgtc gtccttgaag aagatggtgc gctcctggac
      361 gtagccttcg ggcatggcgg acttgaagaa gtcgtgctgc ttcatgtggt cggggtagcg
      421 gctgaagcac tgcacgcccc aggtcagggt ggtcacgagg gtgggccagg gcacgggcag
      481 cttgccggtg gtgcagatga acttcagggt cagcttgccg taggtggcat cgccctcgcc
      541 ctcgccggac acgctgaact tgtggccgtt tacgtcgccg tccagctcga ccaggatggg
      601 caccaccccg gtgaacagct cctcgccctt gctcaccatg aattctttgc caaagtgatg
      661 ggccagcaca cagaccagca cgttgcccag gagctgtggg aggaagataa gaggtatgaa
      721 catgattagc aaaagggcct agcttggact cagaataatc cagccttatc ccaaccataa
      781 aataaaagca gaatggtagc tggattgtag ctgctattag caatatgaaa cctcttacat
      841 cagttacaat ttatatgcag aaatatttat atgcagaaat attgctattg ccttaaccca
      901 gaaattatca ctgttattct ttagaatggt gcaaagaggc atgatacatt gtatcattat
      961 tgccctgaaa gaaagagatt agggaaagta ttagaaataa gataaacaaa aa
//
