LOCUS       sequence-104417-        1137 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Yeast plasmid with Hsp82.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1137)
  AUTHORS   Jiang L, Mishra P, Hietpas RT, Zeldovich KB, Bolon DN
  TITLE     Latent effects of Hsp90 mutants revealed at reduced expression 
            levels.
  JOURNAL   PLoS Genet. 2013 Jun;9(6):e1003600. doi: 
            10.1371/journal.pgen.1003600. Epub 2013 Jun 27.
  PUBMED    23825969
REFERENCE   2  (bases 1 to 1137)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS 
            Genet."; date: "2013-06"; volume: "9(6):e1003600. doi"; pages: " 
            10.1371/journal.pgen.1003600. Epub 2013 Jun 27"
COMMENT     Sequence Label: p414TEF_Hsp82_CycTER
FEATURES             Location/Qualifiers
     source          1..1137
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     203..225
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     217..234
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     218..234
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     244..263
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        244..262
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     terminator      complement(281..528)
                     /gene="S. cerevisiae CYC1"
                     /label=CYC1 terminator
                     /note="transcription terminator for CYC1"
     primer_bind     494..512
                     /label=CYC1
                     /note="CYC1 transcription termination signal, reverse 
                     primer"
ORIGIN
        1 ggcgctggca agtgtagcgg tcacgctgcg cgtaaccacc acacccgccg cgcttaatgc
       61 gccgctacag ggcgcgtcca ttcgccattc aggctgcgca actgttggga agggcgatcg
      121 gtgcgggcct cttcgctatt acgccagctg gcgaaagggg gatgtgctgc aaggcgatta
      181 agttgggtaa cgccagggtt ttcccagtca cgacgttgta aaacgacggc cagtgagcgc
      241 gcgtaatacg actcactata gggcgaattg ggtaccggcc gcaaattaaa gccttcgagc
      301 gtcccaaaac cttctcaagc aaggttttca gtataatgtt acatgcgtac acgcgtctgt
      361 acagaaaaaa aagaaaaatt tgaaatataa ataacgttct taatactaac ataactataa
      421 aaaaataaat agggacctag acttcaggtt gtctaactcc ttccttttcg gttagagcgg
      481 atgtgggggg agggcgtgaa tgtaagcgtg acataactaa ttacatgact cgaatcgatt
      541 ggatccatta atctacctct tccatttcgg tgtcagctgg aacctcttca accggagctg
      601 cggtggatgc ttctggagca gtctctgttt cttcatcctc atcaatgttc aggcctaaag
      661 agatcaatct gttaattctt gatgcaaagg aagttggttc gtccaaactg aagccggaag
      721 tcaacaaagc agtttcatat aataacttag tcaagtcctt gacagtcttg tcttgagcac
      781 caccttcgtc aactctcttt ttcaattcct tgataattgg agattttgga gaaatttcga
      841 aagtcttctt ggaagacatg taggaggaca tggaagagtc tctcaaggct tgagccttca
      901 tgattctttc catgttagca gaccaaccaa attgaccagt tctgatagca gctggggcat
      961 ccaacaattt gtaagaaaca acaactttct ccacttggtc acccaaaatt tctttcaaag
     1021 ctttggtcaa tggttcatat tctttgatct ccttctctct ttcagctttt tcttcgtcag
     1081 tttcttccaa ttcgaaatct ttagtaatgt caaccaaagt tttaccttcg aattcct
//
