LOCUS       sequence-104418-         726 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Transformation vector pBGRY.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 726)
  AUTHORS   Koh TW, He Z, Gorur-Shandilya S, Menuz K, Larter NK, Stewart S, 
            Carlson JR
  TITLE     The Drosophila IR20a clade of ionotropic receptors are candidate 
            taste and pheromone receptors.
  JOURNAL   Neuron. 2014 Aug 20;83(4):850-65. doi: 10.1016/j.neuron.2014.07.012.
            Epub 2014 Aug 7.
  PUBMED    25123314
REFERENCE   2  (bases 1 to 726)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.neuron.2014.07.012"; journalName: "Neuron"; date: 
            "2014-08-20- 20"; volume: "83"; issue: "4"; pages: "850-65"
COMMENT     Sequence Label: pBGRY
FEATURES             Location/Qualifiers
     source          1..726
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        complement(join(79..85,86..103,104..109))
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     protein_bind    complement(134..258)
                     /gene="mutant version of attR"
                     /label=attR1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    305..690
                     /label=gypsy insulator
                     /bound_moiety="Su(Hw), CP190, andMod(mdg4)67.2"
                     /note="chromatin insulator from Drosophila"
ORIGIN
        1 tatatccagt gatttttttc tccattttag cttccttagc tcctgaaaat ctcgacggat
       61 cctaactcaa aatccacaca ttatacgagc cggaagcata aagtgtaaag cctggggtgc
      121 ctaatgcggc cgccatagtg actggatatg ttgtgtttta cagtattatg tagtctgttt
      181 tttatgcaaa atctaattta atatattgat atttatatca ttttacgttt ctcgttcagc
      241 ttttttgtac aaacttgtga taaacgaatt cttgaagacg aaagggccgg ccgtttaaac
      301 tggccacgta ataagtgtgc gttgaattta ttcgcaaaaa cattgcatat tttcggcaaa
      361 gtaaaatttt gttgcatacc ttatcaaaaa ataagtgctg catacttttt agagaaacca
      421 aataattttt tattgcatac ccgtttttaa taaaatacat tgcataccct cttttaataa
      481 aaaatattgc atactttgac gaaacaaatt ttcgttgcat acccaataaa agattattat
      541 attgcatacc cgtttttaat aaaatacatt gcataccctc ttttaataaa aaatattgca
      601 tacgttgacg aaacaaattt tcgttgcata cccaataaaa gattattata ttgcatacct
      661 tttcttgcca taccatttag ccgatcaatt gtgctcggca acagtatatt tgtggtgtgc
      721 caacca
//
