LOCUS       sequence-104420-         851 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  encodes an inert blue fluorescent protein for use in oxidizing 
            cellular environments including the periplasm and mammalian 
            secretory pathway..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 851)
  AUTHORS   Costantini LM, Subach OM, Jaureguiberry-bravo M, Verkhusha VV, Snapp
            EL
  TITLE     Cysteineless non-glycosylated monomeric blue fluorescent protein, 
            secBFP2, for studies in the eukaryotic secretory pathway.
  JOURNAL   Biochem Biophys Res Commun. 2013 Jan 18;430(3):1114-9. doi: 
            10.1016/j.bbrc.2012.12.028. Epub 2012 Dec 19.
  PUBMED    23257162
REFERENCE   2  (bases 1 to 851)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.bbrc.2012.12.028"; journalName: "Biochem Biophys Res 
            Commun"; date: "2013-01-18- 18"; volume: "430"; issue: "3"; pages: 
            "1114-9"
COMMENT     Sequence Label: psecBFP2
FEATURES             Location/Qualifiers
     source          1..851
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     misc_feature    13..93
                     /label=MCS
                     /note="multiple cloning site"
     CDS             107..802
                     /codon_start=1
                     /product="monomeric blue fluorescent protein"
                     /label=TagBFP
                     /note="mammalian codon-optimized"
                     /translation="ELIKENMHMKLYMEGTVDNHHFKSTSEGEGKPYEGTQTMRIKVVE
                     GGPLPFAFDILATSFLYGSKTFIDHTQGIPDFFKQSFPEGFTWERVTTYEDGGVLTATQ
                     DTSLQDGSLIYNVKIRGVDFTSNGPVMQKKTLGWEAFTETLYPADGGLEGRNDMALKLV
                     GGSHLIANIKTTYRSKKPAKNLKMPGVYYVDYRLERIKEANDETYVEQHEVAVARYSDL
                     PSKLGHKLN"
ORIGIN
        1 accgtcagat ccgctagcgc taccggactc agatctcgag ctcaagcttc gaattctgca
       61 gtcgacggta ccgcgggccc gggatccacc ggtcgccagc agcgaggagc tgattaagga
      121 gaacatgcac atgaagctgt acatggaggg caccgtggac aaccatcact tcaagagcac
      181 atccgagggc gaaggcaagc cctacgaggg cacccagacc atgagaatca aggtggtcga
      241 gggcggccct ctccccttcg ccttcgacat cctggctact agcttcctct acggcagcaa
      301 gaccttcatc gaccacaccc agggcatccc cgacttcttc aagcagtcct tccctgaggg
      361 cttcacatgg gagagagtca ccacatacga agacgggggc gtgctgaccg ctacccagga
      421 caccagcctc caggacggca gcctcatcta caacgtcaag atcagagggg tggacttcac
      481 atccaacggc cctgtgatgc agaagaaaac actcggctgg gaggccttca ccgagacgct
      541 gtaccccgct gacggcggcc tggaaggcag aaacgacatg gccctgaagc tcgtgggcgg
      601 gagccatctg atcgcaaaca tcaagaccac atatagatcc aagaaacccg ctaagaacct
      661 caagatgcct ggcgtctact atgtggacta cagactggaa agaatcaagg aggccaacga
      721 cgagacctac gtcgagcagc acgaggtggc agtggccaga tacagcgacc tccctagcaa
      781 actggggcac aagcttaatt aagcggccgc gactctagat cataatcagc cataccacat
      841 ttgtagaggt t
//
