LOCUS       sequence-104421-         827 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  CRISPR/Cas based plant genome editing and gene regulation; expresses
            3×FLAG-NLS-zCas9-NLS, gRNA scaffold for insertion of target sequence
            (TaU3 promoter), Bar resistance.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 827)
  AUTHORS   Xing HL, Dong L, Wang ZP, Zhang HY, Han CY, Liu B, Wang XC, Chen QJ
  TITLE     A CRISPR/Cas9 toolkit for multiplex genome editing in plants.
  JOURNAL   BMC Plant Biol. 2014 Nov 29;14(1):327.
  PUBMED    25432517
REFERENCE   2  (bases 1 to 827)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "BMC Plant 
            Biol."; date: "2014-11-29"; volume: "14(1)"; pages: "327"
COMMENT     Sequence Label: pBUN421
FEATURES             Location/Qualifiers
     source          1..827
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     misc_RNA        228..303
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
ORIGIN
        1 ggcctcgcgc gcagatcagt tggaagaatt tgtccactac gtgaaaggcg agatcaccaa
       61 ggtagtcggc aaataatgtc tagctagaaa ttcgttcaag ccgacgccgc ttcgcggcgc
      121 ggcttaactc aagcgttaga tgcactaagc acataattgc tcacagccaa actatcaggt
      181 caagtctgct tttattattt ttaagcgtgc ataataagcc ggtctcggtt ttagagctag
      241 aaatagcaag ttaaaataag gctagtccgt tatcaacttg aaaaagtggc accgagtcgg
      301 tgcttttttt tttgtccttc tgttttttta gtcagtctct tttttcagaa gtacaacatc
      361 ttttttttgt ccttctgttt ttttagtcag tctctttttt cagaagtaca acatcttttt
      421 tttgtccttc tgttttttta gtcagtctct tttttcagaa gtacaacatc ttttttttgt
      481 ccttctgttt ttttagtcag tctctttttt cagaagtact ctatgatgta tcgttctggg
      541 aaatgtctgt ctgtctacaa ccccataatc tatatttgca atcacacatc taatattctc
      601 tgtgacaaga cagccgaaca agcttgatat cgaattcctg cagtgcagcg tgacccggtc
      661 gtgcccctct ctagagataa tgagcattgc atgtctaagt tataaaaaat taccacatat
      721 tttttttgtc acacttgttt gaagtgcagt ttatctatct ttatacatat atttaaactt
      781 tactctacga ataatataat ctatagtact acaataatat cagtgtt
//
