LOCUS       sequence-104424-         562 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  CRISPR/Cas based plant genome editing and gene regulation; expresses
            3×FLAG-NLS-zCas9-NLS, gRNA scaffold for insertion of target sequence
            (AtU6-26 promoter), Hyg resistance.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 562)
  AUTHORS   Xing HL, Dong L, Wang ZP, Zhang HY, Han CY, Liu B, Wang XC, Chen QJ
  TITLE     A CRISPR/Cas9 toolkit for multiplex genome editing in plants.
  JOURNAL   BMC Plant Biol. 2014 Nov 29;14(1):327.
  PUBMED    25432517
REFERENCE   2  (bases 1 to 562)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "BMC Plant 
            Biol."; date: "2014-11-29"; volume: "14(1)"; pages: "327"
COMMENT     Sequence Label: pHSE401
FEATURES             Location/Qualifiers
     source          1..562
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     misc_RNA        233..308
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
ORIGIN
        1 cgcttggcct cgcgcgcaga tcagttggaa gaatttgtcc actacgtgaa aggcgagatc
       61 accaaggtag tcggcaaata atgtctagct agaaattcgt tcaagccgac gccgcttcgc
      121 ggcgcggctt aactcaagcg ttagatgcac taagcacata attgctcaca gccaaactat
      181 caggtcaagt ctgcttttat tatttttaag cgtgcataat aagccggtct cggttttaga
      241 gctagaaata gcaagttaaa ataaggctag tccgttatca acttgaaaaa gtggcaccga
      301 gtcggtgctt ttttttgcaa aattttccag atcgatttct tcttcctctg ttcttcggcg
      361 ttcaatttct ggggttttct cttcgttttc tgtaactgaa acctaaaatt tgacctaaaa
      421 aaaatctcaa ataatatgat tcagtggttt tgtacttttc agttagttga gttttgcagt
      481 tccgatgaga taaaccaata agcttgcatg cctgcaggtc aacatggtgg agcacgacac
      541 acttgtctac tccaaaaata tc
//
