LOCUS       sequence-104427-         354 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  CRISPR/Cas based plant genome editing and gene regulation; expresses
            3×FLAG-NLS-zCas9-NLS, gRNA scaffold for insertion of target sequence
            (OsU3 promoter), Hyg resistance.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 354)
  AUTHORS   Xing HL, Dong L, Wang ZP, Zhang HY, Han CY, Liu B, Wang XC, Chen QJ
  TITLE     A CRISPR/Cas9 toolkit for multiplex genome editing in plants.
  JOURNAL   BMC Plant Biol. 2014 Nov 29;14(1):327.
  PUBMED    25432517
REFERENCE   2  (bases 1 to 354)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "BMC Plant 
            Biol."; date: "2014-11-29"; volume: "14(1)"; pages: "327"
COMMENT     Sequence Label: pHUE411
FEATURES             Location/Qualifiers
     source          1..354
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     misc_RNA        126..201
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
ORIGIN
        1 gacgccgctt cgcggcgcgg cttaactcaa gcgttagatg cactaagcac ataattgctc
       61 acagccaaac tatcaggtca agtctgcttt tattattttt aagcgtgcat aataagccgg
      121 tctcggtttt agagctagaa atagcaagtt aaaataaggc tagtccgtta tcaacttgaa
      181 aaagtggcac cgagtcggtg cttttttttt tcgttttgca ttgagttttc tccgtcgcat
      241 gtttgcagtt ttattttccg ttttgcattg aaatttctcc gtctcatgtt tgcagcgtgt
      301 tcaaaaagta cgcagctgta tttcacttat ttacggcgcc acattttcat gccg
//
