LOCUS       sequence-104429-         586 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  CRISPR/Cas based plant genome editing and gene regulation; expresses
            3×FLAG-NLS-zCas9-NLS, gRNA scaffold for insertion of target sequence
            (AtU6-26 promoter), Kan resistance.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 586)
  AUTHORS   Xing HL, Dong L, Wang ZP, Zhang HY, Han CY, Liu B, Wang XC, Chen QJ
  TITLE     A CRISPR/Cas9 toolkit for multiplex genome editing in plants.
  JOURNAL   BMC Plant Biol. 2014 Nov 29;14(1):327.
  PUBMED    25432517
REFERENCE   2  (bases 1 to 586)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "BMC Plant 
            Biol."; date: "2014-11-29"; volume: "14(1)"; pages: "327"
COMMENT     Sequence Label: pKSE401
FEATURES             Location/Qualifiers
     source          1..586
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     misc_RNA        174..249
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
ORIGIN
        1 caccaaggta gtcggcaaat aatgtctagc tagaaattcg ttcaagccga cgccgcttcg
       61 cggcgcggct taactcaagc gttagatgca ctaagcacat aattgctcac agccaaacta
      121 tcaggtcaag tctgctttta ttatttttaa gcgtgcataa taagccggtc tcggttttag
      181 agctagaaat agcaagttaa aataaggcta gtccgttatc aacttgaaaa agtggcaccg
      241 agtcggtgct tttttttgca aaattttcca gatcgatttc ttcttcctct gttcttcggc
      301 gttcaatttc tggggttttc tcttcgtttt ctgtaactga aacctaaaat ttgacctaaa
      361 aaaaatctca aataatatga ttcagtggtt ttgtactttt cagttagttg agttttgcag
      421 ttccgatgag ataaaccaat aagcttgcat gcctgcaggt caacatggtg gagcacgaca
      481 cacttgtcta ctccaaaaat atcaaagata cagtctcaga agaccaaagg gcaattgaga
      541 cttttcaaca aagggtaata tccggaaacc tcctcggatt ccattg
//
