LOCUS       sequence-104434-         508 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Part of Multibow set of constructs--each FP gene is initially not 
            expressed and then adopts a permanent ON or OFF status upon 
            Cre-mediated recombination. Encodes membrane bound EBFP2..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 508)
  AUTHORS   Xiong F, Obholzer ND, Noche RR, Megason SG
  TITLE     Multibow: digital spectral barcodes for cell tracing.
  JOURNAL   PLoS One. 2015 May 26;10(5):e0127822. doi: 
            10.1371/journal.pone.0127822. eCollection 2015.
  PUBMED    26010570
REFERENCE   2  (bases 1 to 508)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2015-05-26"; volume: "10(5):e0127822. doi"; pages: " 
            10.1371/journal.pone.0127822. eCollection 2015"
COMMENT     Sequence Label: pMTB-Multibow-mB
FEATURES             Location/Qualifiers
     source          1..508
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    complement(3..36)
                     /label=lox2272
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (AAGTATCC) (Shaw et al., 2021). lox2272 sites are 
                     compatible with each other, but incompatible with loxP or 
                     loxN sites (Lee and Saito, 1988)."
     regulatory      155..164
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     complement(239..260)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
ORIGIN
        1 ggataacttc gtataggata ctttatacga agttattgat caattcacag ccaccatgct
       61 gtgctgcatc agaagaacta aaccggttga gaagaatgaa gaggccgatc aggagctgca
      121 gtcgacggta ccgcgggccc gggatccacc ggtcgccacc atgggagcgc cagctggagg
      181 tgcaggtgca gcaatggtga gcaagggcga ggagctgttc accggggtgg tgcccatcct
      241 ggtcgagctg gacggcgacg taaacggcca caagttcagc gtgaggggcg agggcgaggg
      301 cgatgccacc aacggcaagc tgaccctgaa gttcatctgc accaccggca agctgcccgt
      361 gccctggccc accctcgtga ccaccctgag ccacggcgtg cagtgcttcg cccgctaccc
      421 cgaccacatg aagcagcacg acttcttcaa gtccgccatg cccgaaggct acgtccagga
      481 gcgcaccatc ttcttcaagg acgacggc
//
