LOCUS       sequence-104438-        1001 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Firefly luciferase (Promega) yeast codon-optimized yellow 
            fluorescent protein (yEVenus) fusion with PEST degron driven by SIC1
            promoter..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1001)
  AUTHORS   Mazo-Vargas A, Park H, Aydin M, Buchler NE
  TITLE     Measuring fast gene dynamics in single cells with time-lapse 
            luminescence microscopy
  JOURNAL   Mol Biol Cell. 2014 Sep 17. pii: mbc.E14-07-1187.
  PUBMED    25232010
REFERENCE   2  (bases 1 to 1001)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Biol 
            Cell. 2014 Sep 17. pii: mbc.E14-07-1187."
COMMENT     Sequence Label: pNB785
FEATURES             Location/Qualifiers
     source          1..1001
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     23..43
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        25..43
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(416..434)
                     /label=LucNrev
                     /note="Firefly luciferase, reverse primer"
ORIGIN
        1 tgaccatgat tacgccaagc gcgcaattaa ccctcactaa agggaacaaa agctggagct
       61 cctccggcga tcaaacatac cattcccccc tcaaatcaat atgattaagt cacgataata
      121 tctgcactat tgccgtgttt ccttctccat cgacagcgtc gatcctgtct attatagcca
      181 gcacactgaa aatttcaatg gctggcttgt tgttcgcgtc gcaacttttg atatttataa
      241 aaagagagaa ggcatatcca aaggataagg tattgtcttt gtaaatcaat caaccaaacc
      301 tctacggaat tttgaccctt gaagcaggga ctattacacg ttaattaaca tggaagacgt
      361 caaaaacata aagaaaggcc cggcgccatt ctatccgctg gaagatggaa ccgctggaga
      421 gcaactgcat aaggctatga agagatacgc cctggttcct ggaacaattg cttttacaga
      481 tgcacatatc gaggtggaca tcacttacgc tgagtacttc gaaatgtccg ttcggttggc
      541 agaagctatg aaacgatatg ggctgaatac aaatcacaga atcgtcgtat gcagtgaaaa
      601 ctctcttcaa ttctttatgc cggtgttggg cgcgttattt atcggagttg cagttgcgcc
      661 cgcgaacgac atttataatg aacgtgaatt gctcaacagt atgggcattt cgcagcctac
      721 cgtggtgttc gtttccaaaa aggggttgca aaaaattttg aacgtgcaaa aaaagctccc
      781 aatcatccaa aaaattatta tcatggattc taaaacggat taccagggat ttcagtcgat
      841 gtacacgttc gtcacatctc atctacctcc cggttttaat gaatacgatt ttgtgccaga
      901 gtccttcgat agggacaaga caattgcact gatcatgaac tcctctggat ctactggtct
      961 gcctaaaggt gtcgctctgc ctcatagaac tgcctgcgtg a
//
