LOCUS       sequence-104442-        1055 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Green beetle luciferase with nuclear localization signal (NLS) and 
            ClpXP degron tag (ssrA), driven by LEU1 promoter. .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1055)
  AUTHORS   Mazo-Vargas A, Park H, Aydin M, Buchler NE
  TITLE     Measuring fast gene dynamics in single cells with time-lapse 
            luminescence microscopy
  JOURNAL   Mol Biol Cell. 2014 Sep 17. pii: mbc.E14-07-1187.
  PUBMED    25232010
REFERENCE   2  (bases 1 to 1055)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Biol 
            Cell. 2014 Sep 17. pii: mbc.E14-07-1187."
COMMENT     Sequence Label: pNB753
FEATURES             Location/Qualifiers
     source          1..1055
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     23..43
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        25..43
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     CDS             966..977
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
ORIGIN
        1 tgaccatgat tacgccaagc gcgcaattaa ccctcactaa agggaacaaa agctggagct
       61 ctgatgaacg gtaagccaat gaaggaaaag aagtctacca gaagtgtcga agacttcatg
      121 gctgctatgc aaagagtctc tactcaacac gaaaaggaaa cctaatcctg ttgaagtagc
      181 atttaatcat aatttttgtc acattttaat caacttgatt tttctggttt aatttttcta
      241 attttaattt taattttttt atcaatggga actgatacac taaaaagaat taggagccaa
      301 caagaataag ccgcttattt cctactagag tttgcttaaa atttcatctc gaattgtcat
      361 tctaatattt tatccacaca cacaccttaa aatttttaga ttaaatggca tcaactctta
      421 gcttcacaca cacacacaca ccgaagctgg ttgttttatt tgatttgata taattggttt
      481 ctctggatgg tactttttct ttcttggtta tttcctattt taaaatatga aacgcacaca
      541 agtcataatt attctaatag agcacaattc acaacacgca catttcaact ttaatatttt
      601 tttagaaaca ctttatttag tctaattctt aatttttaat atatataatg cacacacact
      661 aatttattca ttaatttttt attgagtagg atttgaaaat atttggtatc tttgcaagat
      721 gtttgtatag agggacaaag aatcgtcttt attatggtca aggctttacg tcataatagt
      781 tcctgcccag ctcttctata atactttaaa gatctcttct cgtttgctcc atttggaagt
      841 ctcgcttacg tttatgcgcc catacagaca ctcaagatac acacttacat gaacgtatac
      901 aaatttacta acactacttg aaaatatgaa ccacagtaca tcatattaag acgtagtatt
      961 cgatgattga aggccgcctc cgcgaaatac ctttactgat tttgccggtt aatcgcatcg
     1021 aaatttcttc atcacaagaa agcaaacaaa tcgcc
//
