LOCUS       sequence-104447-        1075 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  phageC31 integrase-expressing helper plasmid for Anopheles 
            transgenesis, vasa promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1075)
  AUTHORS   Volohonsky G, Terenzi O, Soichot J, Naujoks DA, Nolan T, Windbichler
            N, Kapps D, Smidler AL, Vittu A, Costa G, Steinert S, Levashina EA, 
            Blandin SA, Marois E
  TITLE     Tools for Anopheles gambiae Transgenesis.
  JOURNAL   G3 (Bethesda). 2015 Apr 13. pii: g3.115.016808. doi: 
            10.1534/g3.115.016808.
  PUBMED    25869647
REFERENCE   2  (bases 1 to 1075)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "G3 
            (Bethesda). 2015 Apr 13. pii: g3.115.016808. doi: 
            10.1534/g3.115.016808."
COMMENT     Sequence Label: pENTR R4-vas2-integrase-R3
FEATURES             Location/Qualifiers
     source          1..1075
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     14..36
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     28..45
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     29..45
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    complement(95..219)
                     /gene="mutant version of attR"
                     /label=attR4
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
ORIGIN
        1 gcatggatgt tttcccagtc acgacgttgt aaaacgacgg ccagtcttaa gctcgggccc
       61 ctacaggtca ctaataccat ctaagtagtt gattcatagt gactggatat gttgtgtttt
      121 acagtattat gtagtctgtt ttttatgcaa aatctaattt aatatattga tatttatatc
      181 attttacgtt tctcgttcaa cttttctata caaagttggt accgggcccc ccgctagcgt
      241 cgacggtatc gatgtagaac gcgagcaaat tcttttcctt ccatgacagc agcagctaca
      301 gtgggaagcc gaacgtcaga cgtgtttgac atgccgaact gggcgggaaa attacagcgt
      361 gcgctttgtt ttcaagcaaa tcacaactcg ctgcaaacaa aaccgttgag aaattgattg
      421 ttttataatt tgtattgtat tttatttgtt ataataaact aaaaagacat actttttgca
      481 tattttatac ataaaaacat acatgcagca ttataaaaca catataaacc ctccctgtag
      541 agtcccgtat cgaaatcttc catcctagtt gcacagtacg acggacgagt aggccgtgtc
      601 cgtgcaaatt ccagctttta gcagtctttt gctcggagca ctcgcggcga gtcggaggtt
      661 tctgctgagg tgcttagcgc taaattagcc aattgctttt gcaagtgaaa taaccagccg
      721 aatagtactt caaaactcag gtaagtgaac tagttttata gaacaaatgt ttgtttgtta
      781 gaagttagtg aagtgtttgt gaaaaaaatc tctcatttcg gcaaaactaa cgtaactgat
      841 ttcaaattga attattgttt tgtgatgtta tattatttca tccagttgat tagtattttc
      901 ttagttatgt tcaaaataca gttaaattaa atttcatttc atttactcat aaaataatct
      961 cttggcttat ttaatttttc tcgaattcgc ttgtattgtt cagtagcacg cgccattcgc
     1021 cctttgtttc attttgtacc tgctcccact aacacactgg cagtgcgaaa caaaa
//
