LOCUS       sequence-104451-        1082 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Riboprobe for in vitro transcription of Plexin A2 (m2).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1082)
  TITLE     Christiana Ruhrberg plasmids
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1082)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: PLEXIN A2 (m2)
FEATURES             Location/Qualifiers
     source          1..1082
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        22..40
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     22..39
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(792..811)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(793..811)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(818..835)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(818..834)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(827..849)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     complement(1062..1081)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
ORIGIN
        1 tgaccatgat tacgccaagc tatttaggtg acactataga atactcaagc tatgcatcaa
       61 gcttggtacc gagctcggat ccactagtaa cggccgccag tgtgctggaa ttcggcttcg
      121 ttgggttggg gtagtagatg aacttggtgt cgttatagat gagtaaggat tgaacattgt
      181 taaagagaaa tccaaactca tctggccgtt ccacagtgtc cagacctggg cggtagtcac
      241 tggtcagaga gggtgccaga caggtgaggg tggttgtgtt taccactgtg catacattga
      301 cagattcttt gccattaaat ttgactcgga ccctgggctc ctgaatgaca tccaagttga
      361 agcctgtgat ggttaggggt gtgtgcccac tagtgatact ccactctggc tcaatacgtt
      421 ggacccgtgg gtcatctata tactcgaact gcagactgct atccacccgg gctctgtcga
      481 cactcacgga gacagggact ggtcctagtc cattggatga tgggggtgaa acacatacaa
      541 tctcattcat tgacctccca tagaattcac aggtctgatt gcccaggtac actgccacac
      601 tgctcccagc accaaggtaa tggcctgtga tggtcaccat ggtacctcct gactctggtc
      661 cccggatcgg gctgagtgac agcacagaag gattcacaaa agtatactgc tggtgggact
      721 tggaagccga attctgcaga tatccatcac actggcggcc gctcgagcat gcatctagag
      781 ggcccaattc gccctatagt gagtcgtatt acaattcact ggccgtcgtt ttacaacgtc
      841 gtgactggga aaaccctggc gttacccaac ttaatcgcct tgcagcacat ccccctttcg
      901 ccagctggcg taatagcgaa gaggcccgca ccgatcgccc ttcccaacag ttgcgcagcc
      961 tgaatggcga atggacgcgc cctgtagcgg cgcattaagc gcggcgggtg tggtggttac
     1021 gcgcagcgtg accgctacac ttgccagcgc cctagcgccc gctcctttcg ctttcttccc
     1081 tt
//
