LOCUS       sequence-104465-        1047 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Cre recombinase-expressing helper plasmid for Anopheles gene 
            engineering, vasa promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1047)
  AUTHORS   Volohonsky G, Terenzi O, Soichot J, Naujoks DA, Nolan T, Windbichler
            N, Kapps D, Smidler AL, Vittu A, Costa G, Steinert S, Levashina EA, 
            Blandin SA, Marois E
  TITLE     Tools for Anopheles gambiae Transgenesis.
  JOURNAL   G3 (Bethesda). 2015 Apr 13. pii: g3.115.016808. doi: 
            10.1534/g3.115.016808.
  PUBMED    25869647
REFERENCE   2  (bases 1 to 1047)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "G3 
            (Bethesda). 2015 Apr 13. pii: g3.115.016808. doi: 
            10.1534/g3.115.016808."
COMMENT     Sequence Label: pENTR L1-vas2-Cre-L4
FEATURES             Location/Qualifiers
     source          1..1047
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     18..40
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     32..49
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     33..49
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    65..164
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
ORIGIN
        1 gctagcatgg atgttttccc agtcacgacg ttgtaaaacg acggccagtc ttaagctcgg
       61 gccccaaata atgattttat tttgactgat agtgacctgt tcgttgcaac aaattgatga
      121 gcaatgcttt tttataatgc caactttgta caaaaaagca ggctggtacc gggccccccg
      181 ctagcgtcga cggtatcgat gtagaacgcg agcaaattct tttccttcca tgacagcagc
      241 agctacagtg ggaagccgaa cgtcagacgt gtttgacatg ccgaactggg cgggaaaatt
      301 acagcgtgcg ctttgttttc aagcaaatca caactcgctg caaacaaaac cgttgagaaa
      361 ttgattgttt tataatttgt attgtatttt atttgttata ataaactaaa aagacatact
      421 ttttgcatat tttatacata aaaacataca tgcagcatta taaaacacat ataaaccctc
      481 cctgtagagt cccgtatcga aatcttccat cctagttgca cagtacgacg gacgagtagg
      541 ccgtgtccgt gcaaattcca gcttttagca gtcttttgct cggagcactc gcggcgagtc
      601 ggaggtttct gctgaggtgc ttagcgctaa attagccaat tgcttttgca agtgaaataa
      661 ccagccgaat agtacttcaa aactcaggta agtgaactag ttttatagaa caaatgtttg
      721 tttgttagaa gttagtgaag tgtttgtgaa aaaaatctct catttcggca aaactaacgt
      781 aactgatttc aaattgaatt attgttttgt gatgttatat tatttcatcc agttgattag
      841 tattttctta gttatgttca aaatacagtt aaattaaatt tcatttcatt tactcataaa
      901 ataatctctt ggcttattta atttttctcg aattcgcttg tattgttcag tagcacgcgc
      961 cattcgccct ttgtttcatt ttgtacctgc tcccactaac acactggcag tgcgaaacaa
     1021 aagccttcgc acgcgttgct ggtatta
//
