LOCUS       sequence-104467-         976 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Cre recombinase-expressing helper plasmid for Anopheles gene 
            engineering, vasa promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 976)
  AUTHORS   Volohonsky G, Terenzi O, Soichot J, Naujoks DA, Nolan T, Windbichler
            N, Kapps D, Smidler AL, Vittu A, Costa G, Steinert S, Levashina EA, 
            Blandin SA, Marois E
  TITLE     Tools for Anopheles gambiae Transgenesis.
  JOURNAL   G3 (Bethesda). 2015 Apr 13. pii: g3.115.016808. doi: 
            10.1534/g3.115.016808.
  PUBMED    25869647
REFERENCE   2  (bases 1 to 976)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "G3 
            (Bethesda). 2015 Apr 13. pii: g3.115.016808. doi: 
            10.1534/g3.115.016808."
COMMENT     Sequence Label: pENTR L1-vas2-Cre-L4
FEATURES             Location/Qualifiers
     source          1..976
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     7..23
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     7..23
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     28..47
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        28..46
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    78..173
                     /gene="mutant version of attL"
                     /label=attL4
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
ORIGIN
        1 agctgccagg aaacagctat gaccatgtaa tacgactcac tataggggat atcgcggccg
       61 ccctgcagct ggatggcaaa taatgatttt attttgactg atagtgacct gttcgttgca
      121 acaaattgat aagcaatgct ttcttataat gccaactttg tatagaaaag ttgggtgctc
      181 gagccattaa cagcagtttt ttttaaaacg atgtatcaac catctaacat gcatatttta
      241 caattttcag gaaaataatc aagctctaat attgtactaa agaaacatat agatatagta
      301 ataatactat agatatagaa atttcaaata ttctcctttt tggaatagtt agacggtcat
      361 ggtggcctat actggctttt ggaatttact gagtttacgc cgccagttac aagggatcgg
      421 tccatttgag gtaagaaccc tcgatgggca tgttgtcaag tctagcagta tgacgtttgt
      481 accatggtac cgctccgtaa attgaatatg ttattagtat tttcttagcc aaacgagaca
      541 taaacgataa gccactagat aaaggataca tcaagatggt gctctaagcg gcccgcgggc
      601 tgataataaa gtttgaagac ccctgaacga tatgttgaca atgaacgggc tttgtgcagt
      661 tacgctcaat tcgtcgttat cttttttgaa acctgggtta gtctaagtac tttgtacaac
      721 taccttcact tgtgtatttt atcctggaat ggacctaaag gtgacgtatt gttagaatac
      781 ttcagttcta gagtatacaa gtgcgaaaaa tcacctttca gtacattaca ttgaataatg
      841 aaagacaatc ggtgcaagct ttcacgaatt atagaataaa agtgcaagtt cttatgtttt
      901 caaagttttc atcaacgttt tattaacgaa caattctttg ctgtgtgtca tgcttcttct
      961 gtgtgtttta catcgt
//
