LOCUS       sequence-104469-         986 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Cre recombinase-expressing helper plasmid for Anopheles gene 
            engineering, vasa promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 986)
  AUTHORS   Volohonsky G, Terenzi O, Soichot J, Naujoks DA, Nolan T, Windbichler
            N, Kapps D, Smidler AL, Vittu A, Costa G, Steinert S, Levashina EA, 
            Blandin SA, Marois E
  TITLE     Tools for Anopheles gambiae Transgenesis.
  JOURNAL   G3 (Bethesda). 2015 Apr 13. pii: g3.115.016808. doi: 
            10.1534/g3.115.016808.
  PUBMED    25869647
REFERENCE   2  (bases 1 to 986)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "G3 
            (Bethesda). 2015 Apr 13. pii: g3.115.016808. doi: 
            10.1534/g3.115.016808."
COMMENT     Sequence Label: pENTR L1-vas2-Cre-L4
FEATURES             Location/Qualifiers
     source          1..986
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(316..336)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             complement(391..417)
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             complement(436..456)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
ORIGIN
        1 atgaagcatg tttagctggc ccaaatgttg ctggatagtt tttactgcca gaccgcgcgc
       61 ctgaagatat agaagataat cgcgaacatc ttcaggttct gcgggaaacc atttccggtt
      121 attcaacttg caccatgccg cccacgaccg gcaaacggac agaagcattt tccaggtatg
      181 ctcagaaaac gcctggcgat ccctgaacat gtccatcagg ttcttgcgaa cctcatcact
      241 cgttgcatcg accggtaatg caggcaaatt ttggtgtacg gtcagtaaat tggacatgcg
      301 gccgcccggg tcttctacct ttctcttctt ttttggaggg ccgctcatga aagggcgaat
      361 tccctggttt tttttgatgt ttttcatacc cgcatagtca ggaacatcgt atgggtacat
      421 aaatcgtggg tcttctactt ttcgtttttt ttttggtggt gctgcatgct gcgcatcggc
      481 cgtagcttcg tcgtctgatg atgatcccat gtttccggat ccgtttcctt tctttattca
      541 ccggaagatg acaattctcc ttatgggaat gtacgattcg agatctgtgt gtctcccgaa
      601 gatgaaaaaa gctgttggtt tcaaccaaaa aacgtgtccg ttgctcgaag cgcgactaac
      661 ggcgactctc tttcgactag caatggcgtc cgcaggcaca aaccgagagg aatagtggaa
      721 catgcaatgc agcagcagca gcaacagcag cagcacgtgg agagagagaa agaaagatgg
      781 tcgaaatatt acaaaaaata attgccgttg cacacacaca cacacacaca cacacacaca
      841 cacacacaca cacacacaca cacacacaca cacacacaca cacacacaca cacacacaca
      901 cacactacac caccaatatc gacgaccacc gctctacccg cacccattgc aacctaaaga
      961 gacgatggta ttttccagcg aaattt
//
