LOCUS       sequence-104470-         855 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Anopheles docking site transgenesis plasmid expressing Cre 
            recombinase and I-SceI nuclease, vasa promoter, DsRed marker.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 855)
  AUTHORS   Volohonsky G, Terenzi O, Soichot J, Naujoks DA, Nolan T, Windbichler
            N, Kapps D, Smidler AL, Vittu A, Costa G, Steinert S, Levashina EA, 
            Blandin SA, Marois E
  TITLE     Tools for Anopheles gambiae Transgenesis.
  JOURNAL   G3 (Bethesda). 2015 Apr 13. pii: g3.115.016808. doi: 
            10.1534/g3.115.016808.
  PUBMED    25869647
REFERENCE   2  (bases 1 to 855)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "G3 
            (Bethesda). 2015 Apr 13. pii: g3.115.016808. doi: 
            10.1534/g3.115.016808."
COMMENT     Sequence Label: pDSAR-Cre-F2A-I-SceI
FEATURES             Location/Qualifiers
     source          1..855
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(312..332)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             complement(387..413)
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             complement(432..452)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
ORIGIN
        1 agcatgttta gctggcccaa atgttgctgg atagttttta ctgccagacc gcgcgcctga
       61 agatatagaa gataatcgcg aacatcttca ggttctgcgg gaaaccattt ccggttattc
      121 aacttgcacc atgccgccca cgaccggcaa acggacagaa gcattttcca ggtatgctca
      181 gaaaacgcct ggcgatccct gaacatgtcc atcaggttct tgcgaacctc atcactcgtt
      241 gcatcgaccg gtaatgcagg caaattttgg tgtacggtca gtaaattgga catgcggccg
      301 cccgggtctt ctacctttct cttctttttt ggagggccgc tcatgaaagg gcgaattccc
      361 tggttttttt tgatgttttt catacccgca tagtcaggaa catcgtatgg gtacataaat
      421 cgtgggtctt ctacttttcg tttttttttt ggtggtgctg catgctgcgc atcggccgta
      481 gcttcgtcgt ctgatgatga tcccatgttt ccggatccgt ttcctttctt tattcaccgg
      541 aagatgacaa ttctccttat gggaatgtac gattcgagat ctgtgtgtct cccgaagatg
      601 aaaaaagctg ttggtttcaa ccaaaaaacg tgtccgttgc tcgaagcgcg actaacggcg
      661 actctctttc gactagcaat ggcgtccgca ggcacaaacc gagaggaata gtggaacatg
      721 caatgcagca gcagcagcaa cagcagcagc acgtggagag agagaaagaa agatggtcga
      781 aatattacaa aaaataattg ccgttgcaca cacacacaca cacacacaca cacacacaca
      841 cacacacaca cacac
//
