LOCUS       sequence-104471-         844 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Anopheles docking site transgenesis plasmid expressing Cre 
            recombinase and I-SceI nuclease, vasa promoter, DsRed marker.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 844)
  AUTHORS   Volohonsky G, Terenzi O, Soichot J, Naujoks DA, Nolan T, Windbichler
            N, Kapps D, Smidler AL, Vittu A, Costa G, Steinert S, Levashina EA, 
            Blandin SA, Marois E
  TITLE     Tools for Anopheles gambiae Transgenesis.
  JOURNAL   G3 (Bethesda). 2015 Apr 13. pii: g3.115.016808. doi: 
            10.1534/g3.115.016808.
  PUBMED    25869647
REFERENCE   2  (bases 1 to 844)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "G3 
            (Bethesda). 2015 Apr 13. pii: g3.115.016808. doi: 
            10.1534/g3.115.016808."
COMMENT     Sequence Label: pDSAR-Cre-F2A-I-SceI
FEATURES             Location/Qualifiers
     source          1..844
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             199..264
                     /codon_start=1
                     /product="2A peptide from foot-and-mouth disease virus 
                     polyprotein"
                     /label=F2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="VKQTLNFDLLKLAGDVESNPGP"
     CDS             316..336
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             355..381
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
ORIGIN
        1 ctggcctggt ctggacacag tgcccgtgtc ggagccgcgc gagatatggc ccgcgctgga
       61 gtttcaatac cggagatcat gcaagctggt ggctggacca atgtaaatat tgtcatgaac
      121 tatatccgta acctggatag tgaaacaggg gcaatggtgc gcctgctgga agatggcgat
      181 cgcgccaagc gtgctccggt gaagcagacc ctgaacttcg acctgctgaa gctggctgga
      241 gacgtggagt cgaatcccgg accaggatca tcatcagacg acgaagctac ggccgatgcg
      301 cagcatgcag caccaccaaa aaaaaaacga aaagtagaag acccacgatt tatgtaccca
      361 tacgatgttc ctgactatgc gggtatgaaa aacatcaaaa aaaaccaggt aatgaacctg
      421 ggtccgaact ctaaactgct gaaagaatac aaatcccagc tgatcgaact gaacatcgaa
      481 cagttcgaag caggtatcgg tctgatcctg ggtgatgctt acatccgttc tcgtgatgaa
      541 ggtaaaacct actgtatgca gttcgagtgg aaaaacaaag catacatgga ccacgtatgt
      601 ctgctgtacg atcagtgggt actgtccccg ccgcacaaaa aagaacgtgt taaccacctg
      661 ggtaacctgg taatcacctg gggcgcccag actttcaaac accaagcttt caacaaactg
      721 gctaacctgt tcatcgttaa caacaaaaaa accatcccga acaacctggt tgaaaactac
      781 ctgaccccga tgtctctggc atactggttc atggatgatg gtggtaaatg ggattacaac
      841 aaaa
//
