LOCUS       sequence-104473-         934 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Anopheles docking site transgenesis plasmid expressing Cre 
            recombinase and I-SceI nuclease, vasa promoter, DsRed marker.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 934)
  AUTHORS   Volohonsky G, Terenzi O, Soichot J, Naujoks DA, Nolan T, Windbichler
            N, Kapps D, Smidler AL, Vittu A, Costa G, Steinert S, Levashina EA, 
            Blandin SA, Marois E
  TITLE     Tools for Anopheles gambiae Transgenesis.
  JOURNAL   G3 (Bethesda). 2015 Apr 13. pii: g3.115.016808. doi: 
            10.1534/g3.115.016808.
  PUBMED    25869647
REFERENCE   2  (bases 1 to 934)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "G3 
            (Bethesda). 2015 Apr 13. pii: g3.115.016808. doi: 
            10.1534/g3.115.016808."
COMMENT     Sequence Label: pDSAR-Cre-F2A-I-SceI
FEATURES             Location/Qualifiers
     source          1..934
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      40..49
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     protein_bind    564..633
                     /label=attB
                     /bound_moiety="phage phi-C31 integrase"
                     /note="attB site for the phi-C31 integrase (Groth et al., 
                     2000)"
     primer_bind     complement(662..681)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     polyA_signal    691..773
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     717..736
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
ORIGIN
        1 agcgcatgaa ctccttgatg acgttcttgg aggagcgcac catggtggcg accggtggat
       61 cccgggcccg cggtaccgtc gactctagcg gtaccccgat tgtttagctt gttcagctgc
      121 gcttgtttat ttgcttagct ttcgcttagc gacgtgttca ctttgcttgt ttgaattgaa
      181 ttgtcgctcc gtagacgaag cgcctctatt tatactccgg cggtcgaggg ttcgaaatcg
      241 ataagcttgg atcctaattg aattagctct aattgaatta gtctctaatt gaattagatc
      301 cccgggcgag ctcgaattaa ccattgtggg aaccgtgcga tcaaacaaac gcgagatacc
      361 ggaagtctgc aggaattgcc cgccgtgacc gtcgagaacc cgctgacgct gccccgcgta
      421 tccgcacccg ccgacgccgt cgcacgtccc gtgctcaccg tgaccaccgc gcccagcggt
      481 ttcgagggcg agggcttccc ggtgcgccgc gcgttcgccg ggatcaacta ccgccacctc
      541 gacccgttca tcatgatgga ccagatgggt gaggtggagt acgcgcccgg ggagcccaag
      601 ggcacgccct ggcacccgca ccgcggcttc gagactgtga cctacatcgt cgagatacat
      661 tgatgagttt ggacaaacca caactagaat gcagtgaaga aaaatgcttt atttgtgaaa
      721 tttgtgatgc tattgcttta tttgtaacca ttataagctg caataaacaa gttaacaaca
      781 acaattgcat tcattttatg tttcaggttc agggggaggt gtgggaggtt ttttaaagca
      841 agtaaaacct ctacaaatgt ggtatggctg attatgtcta gcgtcgacgg tatcgatgta
      901 gaacgcgagc aaattctttt ccttccatga cagc
//
