LOCUS       sequence-104474-         919 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Anopheles docking site transgenesis plasmid expressing Cre 
            recombinase and I-SceI nuclease, vasa promoter, DsRed marker.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 919)
  AUTHORS   Volohonsky G, Terenzi O, Soichot J, Naujoks DA, Nolan T, Windbichler
            N, Kapps D, Smidler AL, Vittu A, Costa G, Steinert S, Levashina EA, 
            Blandin SA, Marois E
  TITLE     Tools for Anopheles gambiae Transgenesis.
  JOURNAL   G3 (Bethesda). 2015 Apr 13. pii: g3.115.016808. doi: 
            10.1534/g3.115.016808.
  PUBMED    25869647
REFERENCE   2  (bases 1 to 919)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "G3 
            (Bethesda). 2015 Apr 13. pii: g3.115.016808. doi: 
            10.1534/g3.115.016808."
COMMENT     Sequence Label: pDSAR-Cre-F2A-I-SceI
FEATURES             Location/Qualifiers
     source          1..919
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     7..23
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     7..23
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     28..47
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        28..46
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    77..176
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     polyA_signal    237..358
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(248..267)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     302..321
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     complement(541..564)
                     /label=DsRed1-C
                     /note="DsRed1, forward primer"
     primer_bind     complement(739..758)
                     /label=mCherry-F
                     /note="mCherry, forward primer"
ORIGIN
        1 agctgccagg aaacagctat gaccatgtaa tacgactcac tataggggat atcgcggccg
       61 ccctgcagct ggatggcaaa taatgatttt attttgactg atagtgacct gttcgttgca
      121 acaaattgat aagcaatgct ttcttataat gccaactttg tacaagaaag ctgggtactc
      181 gagctccacc gcggtggcgg ccgctctagg cgcgccgtac gcgtatcgat aagctttaag
      241 atacattgat gagtttggac aaaccacaac tagaatgcag tgaaaaaaat gctttatttg
      301 tgaaatttgt gatgctattg ctttatttgt aaccattata agctgcaata aacaagttaa
      361 caacaacaat tgcattcatt ttatgtttca ggttcagggg gaggtgtggg aggtttttta
      421 aagcaagtaa aacctctaca aatgtggtat ggctgattat gatctagagt cgcggccgct
      481 acaggaacag gtggtggcgg ccctcggtgc gctcgtactg ctccacgatg gtgtagtcct
      541 cgttgtggga ggtgatgtcc agcttggagt ccacgtagta gtagccgggc agctgcacgg
      601 gcttcttggc catgtagatg gacttgaact ccaccaggta gtggccgccg tccttcagct
      661 tcagggcctt gtggatctcg cccttcagca cgccgtcgcg ggggtacagg cgctcggtgg
      721 aggcctccca gcccatggtc ttcttctgca ttacggggcc gtcggagggg aagttcacgc
      781 cgatgaactt caccttgtag atgaagcagc cgtcctgcag ggaggagtcc tgggtcacgg
      841 tcaccacgcc gccgtcctcg aagttcatca cgcgctccca cttgaagccc tcggggaagg
      901 acagcttctt gtagtcggg
//
