LOCUS       sequence-10448-e        4677 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4677)
  AUTHORS   Lynch MD, Gill RT
  TITLE     Broad host range vectors for stable genomic library construction.
  JOURNAL   Biotechnol Bioeng. 2006 May 5. 94(1):151-8.
  PUBMED    16496398
REFERENCE   2  (bases 1 to 4677)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Biotechnol 
            Bioeng. 2006 May 5. 94(1):151-8."
COMMENT     Sequence Label: pBTL-6
FEATURES             Location/Qualifiers
     source          1..4677
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      complement(60..96)
                     /label=soxR terminator
                     /note="bidirectional E. coli soxR transcription terminator"
     rep_origin      102..871
                     /label=pBBR1 oriV
                     /note="replication origin of the broad-host-range plasmid 
                     pBBR1 from Bordetella bronchiseptica; requires the pBBR1 
                     Rep protein for replication"
     CDS             872..1534
                     /codon_start=1
                     /product="replication protein for the broad-host-range 
                     plasmid pBBR1 from Bordetella bronchiseptica"
                     /label=pBBR1 Rep
                     /translation="MATQSREIGIQAKNKPGHWVQTERKAHEAWAGLIARKPTAAMLLH
                     HLVAQMGHQNAVVVSQKTLSKLIGRSLRTVQYAVKDLVAERWISVVKLNGPGTVSAYVV
                     NDRVAWGQPRDQLRLSVFSAAVVVDHDDQDESLLGHGDLRRIPTLYPGEQQLPTGPGEE
                     PPSQPGIPGMEPDLPALTETEEWERRGQQRLPMPDEPCFLDDGEPLEPPTRVTLPRR"
     terminator      complement(4522..4553)
                     /label=tonB terminator
                     /note="bidirectional E. coli tonB-P14 transcription 
                     terminator"
     promoter        join(4600..4605,4606..4623,4624..4630)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    4638..4654
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
ORIGIN
        1 aacgaattca agcttgatat cattcaggac gagcctcaga ctccagcgta actggactga
       61 aaacaaacta aagcgccctt gtggcgcttt agttttgttc cgcggccacc ggctggctcg
      121 cttcgctcgg cccgtggaca accctgctgg acaagctgat ggacaggctg cgcctgccca
      181 cgagcttgac cacagggatt gcccaccggc tacccagcct tcgaccacat acccaccggc
      241 tccaactgcg cggcctgcgg ccttgcccca tcaatttttt taattttctc tggggaaaag
      301 cctccggcct gcggcctgcg cgcttcgctt gccggttgga caccaagtgg aaggcgggtc
      361 aaggctcgcg cagcgaccgc gcagcggctt ggccttgacg cgcctggaac gacccaagcc
      421 tatgcgagtg ggggcagtcg aaggcgaagc ccgcccgcct gccccccgag cctcacggcg
      481 gcgagtgcgg gggttccaag ggggcagcgc caccttgggc aaggccgaag gccgcgcagt
      541 cgatcaacaa gccccggagg ggccactttt tgccggaggg ggagccgcgc cgaaggcgtg
      601 ggggaacccc gcaggggtgc ccttctttgg gcaccaaaga actagatata gggcgaaatg
      661 cgaaagactt aaaaatcaac aacttaaaaa aggggggtac gcaacagctc attgcggcac
      721 cccccgcaat agctcattgc gtaggttaaa gaaaatctgt aattgactgc cacttttacg
      781 caacgcataa ttgttgtcgc gctgccgaaa agttgcagct gattgcgcat ggtgccgcaa
      841 ccgtgcggca ccctaccgca tggagataag catggccacg cagtccagag aaatcggcat
      901 tcaagccaag aacaagcccg gtcactgggt gcaaacggaa cgcaaagcgc atgaggcgtg
      961 ggccgggctt attgcgagga aacccacggc ggcaatgctg ctgcatcacc tcgtggcgca
     1021 gatgggccac cagaacgccg tggtggtcag ccagaagaca ctttccaagc tcatcggacg
     1081 ttctttgcgg acggtccaat acgcagtcaa ggacttggtg gccgagcgct ggatctccgt
     1141 cgtgaagctc aacggccccg gcaccgtgtc ggcctacgtg gtcaatgacc gcgtggcgtg
     1201 gggccagccc cgcgaccagt tgcgcctgtc ggtgttcagt gccgccgtgg tggttgatca
     1261 cgacgaccag gacgaatcgc tgttggggca tggcgacctg cgccgcatcc cgaccctgta
     1321 tccgggcgag cagcaactac cgaccggccc cggcgaggag ccgcccagcc agcccggcat
     1381 tccgggcatg gaaccagacc tgccagcctt gaccgaaacg gaggaatggg aacggcgcgg
     1441 gcagcagcgc ctgccgatgc ccgatgagcc gtgttttctg gacgatggcg agccgttgga
     1501 gccgccgaca cgggtcacgc tgccgcgccg gtagtacgta agaggttcca actttcacca
     1561 taatgaaata agatcactac cgggcgtatt ttttgagtta tcgagatttt caggagctaa
     1621 ggaagctaaa atggaagaac aaagcgtgaa catggcgcga ttgaaggggg aggttttgcc
     1681 cgccctcttc gcgtcgccgg cgacgattgg cgagtacggg gccggcatcg acggggcgga
     1741 ttccctcaac gagctgtcga atctgatgga gcacggcgca gttgccgcgc tggccgacaa
     1801 aatcagccag atcgtggcga agctggccga cgcggacccc cgcaagatcg cggaaaagcc
     1861 tacctggttc gagaagatgc ttggccgtga ggttgaacgc caggtgaggt atcaggtcgc
     1921 ccgcaagacg ctcgaccagt tgctggacga agccgagggc gtagcgcagc gcgtgcggga
     1981 cacgttgcgc gccttggatg acatgctcaa tacgcatgag gccgaggtag accggctcag
     2041 agcctacatt caagccgggc gcgagttcct ggacgagaac cccgaggccg gcgcggccaa
     2101 ggccggcgtg atcgagttcg acaagccgcg cgaacgcttc gcgcgcaagc tcgccaacct
     2161 ggcaaccctc atggcgtccc atgaaatgag cgtcactcag atgaagctca cgcgggcgca
     2221 ggccgtggac atgctggacc gcttctctga aacggcatcc gtcctggtgc ccgtctggcg
     2281 tcagcacacc ctcgcgctca tcaccaccaa gaacatgaat ccggcaatgg tcgccgaggc
     2341 ggccaaagct caccaggcgc tcatgcggag cctttcgcag agcctggaag gcatcaacca
     2401 ataacacggc gggagaaccc tatgaacgca ctgaaaacga cgcacgacgc caaggcccct
     2461 atcgtcgcct tcgacatgac cccggcaacc ctgcgcgagc tgggcttgca ggaaagcgac
     2521 gtgccggaag tccatgcggt cgcgcagcgg atcgaggtcg gcagtccgca gaccgttgcc
     2581 gagttcggcc gcgacgtggc cgagcacacg tcccgctacg ccgatagcct gctggaccag
     2641 gtgcgcaaca gcgacctgga cgaagcaggc gagaaactga cccaggttgt cgccaaggcc
     2701 cgttccctga acgtcggccc tttgtccgac aaccgttccc gcctgcccct gattggcccg
     2761 ctgatcgacc gcttccgcgt ccgttcgacg ggcttcatgg cgcgcttcga cacgacccgc
     2821 gagcagatcg aacacctggt cagcgaagtg cagaccaccc agcaaggcat cgcgcagcgc
     2881 aatgcctcgc tcgacgaaat gttcgcagcc gtgcgcgagg aacaccgcct tcttggcgtc
     2941 cacatcgcgg ccggcaaggt ccgccttgcc gagctgcgcg agcaggccga gggtctgcgc
     3001 ggcaatgtcg ggaacgaccc gggccgcgtg caggagctgg ccgacctcga tgcgatggtt
     3061 gccaacctgg acaagcgcat cggcgacctg atcgccttgc aacattcggc catgcagagc
     3121 ctgccgacca tccgcatgat ccaggccaac aaccagatgc tggtcgataa attccacacc
     3181 atccgcgaaa tcaccgtgcc ggcgtggaag cggcaattca tgctggcctt gagcctcaac
     3241 gagcagaaga acgccgtcga actggccacg gccatcgacg acaccaccaa cgacctgatg
     3301 aagcgcaatg cggccctgct gcatcgcacg tccgtcgaga cggcgaagga gaaccaacgc
     3361 ctggtgatcg acgtggacac gctcaagcag gttcagacga cgctcatcaa gaccgtcgag
     3421 gacgttattc gcatccagca ggaaggcgtg cagaagcgca aggatgccga gaagcagatc
     3481 gccgcaatgc gtggcgatct tcaagccaag ctgacccgcc agcccgtgcg cgagctggcc
     3541 caacaggagt ccgtatgaat gccacaaaca ccgatgtttt cgcccaggta ggcggcctcg
     3601 aggcccgagg cgcgaagatg aagaagcggg gcacccgctt cctcatcgcg gcgctggcag
     3661 tccttgccat tgccgggatc ggggcagtaa cgggatgggc gatcagcccg agcgcgacgc
     3721 ccggaagcat tgacgtgccg caggtgctgg catcgacatt cagcgaccag gtgccgggca
     3781 gtgagggcgg cggcctgggt ggcggcctgc ccttcacttc ggccgtcggg gcattcacgg
     3841 acttcatggc ggggccggca atttttacct tgggcattct tggcatagtg gtcgcgggtg
     3901 ccgtgctcgt gttcgggggt gaattctgcg ggttcgtgcg atccgtctgc atgatggtga
     3961 tagccgtcag catgattttc gtgtcgtcga acttggtgaa gggcattctc ggcggcgatc
     4021 acgacgccgg ccctgcggag ccttcgccgc gtgcgcgatt catggcggcc gtggaggcca
     4081 aggatttcgc gcgagtgcaa gagctgatcg aggcgcgtgg agccaagtcg gcggctgatt
     4141 atgtccttgc gcagctcgcc gtggccgaag gtctggaccg caagcctggt gcgcgcgtcg
     4201 tggtcgggaa agcggcgggc agcatggcaa tgccgcctgc ggcgctgggt tttacgccaa
     4261 ggggagaagc ggcatacgcc atcgagcggt cagcctatgg tgagccgagg tccagcattg
     4321 cgaagcagta ccagcaggaa tggaaccgga aggcggcgac ctggtgggcg atggccggtg
     4381 tggccggcat catcggcgcg atcctggcgg cggcggcaac cggctttgtt gggctggcag
     4441 tgtcgatccg caaccgagtg aagcgcgtgc gcgacctgtt ggtgatggag ccgggtgcag
     4501 agccataagg gatccccctc aagtcaaaag cctccggtcg gaggcttttg actttctgct
     4561 atggaggtca ggtatgattt tgcattaggc accccaggct ttacacttta tgcttccggc
     4621 tcgtatgttg tgtggaattg tgagcggata acaatttcac actctagaga attcgtc
//
